Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy
Applied Optics, Vol. 48, Issue 16, pp. 2983-2990 (2009)
http://dx.doi.org/10.1364/AO.48.002983
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Abstract
Measurement of blood flow with high spatial and temporal resolutions in a three-dimensional (3D) volume is a challenge in biomedical research fields. In this study, digital holographic microscopy is used to measure the 3D motion of human red blood cells (RBCs) in a microscale volume. The cinematographic holography technique, which uses a high-speed camera, enabled the continuous tracking of individual RBCs in a microtube flow. Several autofocus functions that quantify the sharpness of reconstructed RBC images are evaluated to locate the accurate depthwise position of RBCs. In this study, the squared Laplacian function yields the smallest depth of focus and locates the depthwise positions of RBCs with a root mean square error of
© 2009 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
ToC Category:
Holography
History
Original Manuscript: March 4, 2009
Revised Manuscript: April 27, 2009
Manuscript Accepted: April 29, 2009
Published: May 20, 2009
Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Yong-Seok Choi and Sang-Joon Lee, "Three-dimensional volumetric measurement of red blood cell motion using digital holographic microscopy," Appl. Opt. 48, 2983-2990 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-16-2983
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