Laser-induced fluorescence is used to investigate fluorescence properties of unwashed Bacillus thuringiensis and Bacillus subtilis spores, ovalbumin, and washed bacteriophage MS2. A fluorescence detector is calibrated to obtain absolute fluorescence cross sections. Fluorescence maps of biological aerosols and suspensions are measured at a wide excitation range from 210 to
and a wide detection range from 315 to
. The dominant features of the measured spectra are the amino acid peaks, having excitation maxima at 220 and
. The peaks are similar for the bacterial spores, both for aerosols and suspensions, whereas the peaks are shifted toward the shorter emission wavelengths for the suspended ovalbumin and MS2. Moreover, the fluorescence emission, excited above
is more intensive for the aerosols than the suspensions.
© 2009 Optical Society of America
(010.1100) Atmospheric and oceanic optics : Aerosol detection
(300.2530) Spectroscopy : Fluorescence, laser-induced
Atmospheric and Oceanic Optics
Original Manuscript: March 10, 2009
Revised Manuscript: June 23, 2009
Manuscript Accepted: July 7, 2009
Published: July 21, 2009
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics
Albert Manninen, Matti Putkiranta, Jaakko Saarela, Antti Rostedt, Tapio Sorvajärvi, Juha Toivonen, Marko Marjamäki, Jorma Keskinen, and Rolf Hernberg, "Fluorescence cross sections of bioaerosols and suspended biological agents," Appl. Opt. 48, 4320-4328 (2009)
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