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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 22 — Aug. 1, 2009
  • pp: 4354–4364

Measurement of signal intensity depth profiles in rat brains with cardiac arrest using wide-field optical coherence tomography

Manabu Sato, Molly Subhash Hrebesh, and Izumi Nishidate  »View Author Affiliations

Applied Optics, Vol. 48, Issue 22, pp. 4354-4364 (2009)

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Three-dimensional (3D) optical coherence tomography (OCT) images of rat brain taken through the thinned skull were measured using quadrature fringe wide-field OCT (QF WF OCT) with a period of 10 min for total measurement time of 210 min stopping blood flow due to cardiac arrest, in order to investigate the potential of OCT to monitor tissue viability in brains. First, spatial resolution degradation was evaluated with QF WF OCT to demonstrate that the axial resolution was 390 μm at a thickness of 1000 μm . After cardiac arrest, the signal intensity in depth profiles increased 2.7 times compared with that before cardiac arrest. The ratio of signal intensity after euthanasia with an injection of pentobarbital sodium salt to that before sharply increased for 20 min , with stationary values of 2 to 4 overall. The trends of time variations of each position were similar. However, each stationary value depended on the 3D position.

© 2009 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 10, 2009
Manuscript Accepted: July 6, 2009
Published: July 22, 2009

Virtual Issues
Vol. 4, Iss. 10 Virtual Journal for Biomedical Optics

Manabu Sato, Molly Subhash Hrebesh, and Izumi Nishidate, "Measurement of signal intensity depth profiles in rat brains with cardiac arrest using wide-field optical coherence tomography," Appl. Opt. 48, 4354-4364 (2009)

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