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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 29 — Oct. 10, 2009
  • pp: 5528–5536

Frequency-domain fluorescence lifetime optrode system design and instrumentation without a concurrent reference light-emitting diode

Mohammad Rameez Chatni, Gang Li, and David Marshall Porterfield  »View Author Affiliations


Applied Optics, Vol. 48, Issue 29, pp. 5528-5536 (2009)
http://dx.doi.org/10.1364/AO.48.005528


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Abstract

We report the design, development, and implementation of an improved instrumentation approach for frequency-domain fluorescence lifetime (FDFL) optrodic sensing without a concurrent reference LED. FDFL traditionally uses a reference LED, at approximately the same wavelength as the sensor fluorophore emission, to measure phase shifts associated with changes in the fluorescence lifetime of fluorophore. For this work we used an oxygen optrode to design, develop, and test the reference-LED-free FDFL approach. Electronics and optics were optimized, and key system parameters, such as inherent system phase shifts, were determined to insure best performance. In our tests with the oxygen optrode, we observed that several key performance characteristics were improved by the implementation of the reference-LED-free instrumentation platform. This system can potentially be adapted to other analyte-selective fluorophores, which will enable scientists and researchers to expand the application of optrodic sensors as basic research tools in biology, medicine, and agriculture.

© 2009 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 9, 2009
Revised Manuscript: August 4, 2009
Manuscript Accepted: September 11, 2009
Published: October 5, 2009

Virtual Issues
Vol. 4, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Mohammad Rameez Chatni, Gang Li, and David Marshall Porterfield, "Frequency-domain fluorescence lifetime optrode system design and instrumentation without a concurrent reference light-emitting diode," Appl. Opt. 48, 5528-5536 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-29-5528


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References

  1. S. M. Borisov and O. S. Wolfbeis, “Optical biosensors,” Chem. Rev. 108, 423-461 (2008). [CrossRef]
  2. O. S. Wolfbeis, “Fiber optic chemical sensors and biosensors,” Anal. Chem. 76, 3269-3284 (2004). [CrossRef]
  3. A. ul Haque, M. R. Chatni, G. Li, and D. M. Porterfield, “Biochips and other microtechnologies for physiomics,” Expert Rev. Proteomics 4, 553-563 (2007). [CrossRef]
  4. J. R. Lackowicz, Principles of Fluorescence Spectroscopy (Springer, 2006).
  5. K. Carlsson and A. Liljeborg, “Simultaneous confocal lifetime imaging of multiple imaging of fluorophores using the intensity-modulated multiple-wavelength scanning (IMS) technique,” J. Microsc. 191, 119-127 (1998). [CrossRef]
  6. B. Chance, M. Cope, E. Gratton, N. Ramanujam, and B. Tromberg, “Phase measurement of light absorption and scatter in human tissue,” Rev. Sci. Instrum. 69, 3457-3481(1998). [CrossRef]
  7. J. E. Cantle, Atomic Absorption Spectrometry (Elsevier, 1982).
  8. D. M. Porterfield, J. L. Rickus, and R. Kopelmann, “Non-invasive approaches to measuring respiratory patterns using a PtTFPP based phase-lifetime self-referencing oxygen optrode,” Proc. SPIE 6380, 63800S (2006). [CrossRef]
  9. M. R. Chatni, D. E. Maier, and D. M. Porterfield, “Evaluation of microparticle materials for enhancing the performance of fluorescence lifetime based optrodes,” Sens. Actuators B 141, 471-477 (2009). [CrossRef]
  10. M. J. O'Brien, E. Rabinovich, S. R. J. Brueck, and G. P. Lopez “Technique for detecting changes in fluorescence lifetime by means of optoelectronic circuit auto-oscillation,” Opt. Lett. 26, 1256-1258 (2001). [CrossRef]
  11. O. S. Knudsen, Biological Membranes: Theory of Transport Potentials and Electric Impulses (Cambridge U. Press, 2003).
  12. T. Mock, G. S. Dieckmann, C. Haas, A. Krell, J. L. Tison, A. L. Belem, S. Papadimitriou, and D. N. Thomas, “Micro-optrodes in sea-ice: a new approach to investigate oxygen dynamics during sea ice formation,” Aquat. Microb. Ecol. 29, 297-306 (2002). [CrossRef]
  13. B. C. Sanchez, H. Ochoa-Acuna, D. M. Porterfield, and M. S. Sepulvada, “Oxygen flux as an indicator of physiological stress in fathead minnow embryos: a real-time biomonitoring system of water quality,” Environ. Sci. Technol. 42, 7010-7017(2008). [CrossRef]
  14. K. Kolasinski, Surface Science: Foundations of Catalysis and Nanoscience (Wiley, 2002).
  15. J. N. Demas, B. A. DeGraff, and W. Xu, “Modeling of luminescence quenching-based sensors: comparison of multi-site and non-linear gas solubility models,” Anal. Chem. 67, 1377-1380(1995). [CrossRef]
  16. V. I. Ogurtsov and D. B. Papkovsky, “Approximation of calibration for phase-fluorometric oxygen sensors on the basis of physical models,” Sens. Actuators B 88, 89-100(2003). [CrossRef]
  17. K. A. Kneas, J. N. Demas, B. A. DeGraff, and A. Periasamy, “Fluorescence microscopy study of heterogeneity in polymer-supported luminescence-based oxygen sensors,” Microsc. Microanal. 6, 551-561 (2000).
  18. E. R. Carraway, J. N. Demas, B. A. DeGraff, and R. Bacon, “Photophysics and oxygen quenching of transition-metal complexes on fumed silica,” Langmuir 7, 2991-2998 (1991). [CrossRef]

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