Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source
Applied Optics, Vol. 48, Issue 3, pp. 553-559 (2009)
http://dx.doi.org/10.1364/AO.48.000553
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Abstract
We present the instrumental development of a versatile total internal reflection fluorescence lifetime imaging microscopy setup illuminated by a supercontinuum laser source. It enables performing wide-field fluorescence lifetime imaging with subwavelength axial resolution for a large range of fluorophores. The short overall acquisition time and the axial resolution are well suited for dynamic neurobiological applications.
© 2009 Optical Society of America
OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(260.6970) Physical optics : Total internal reflection
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: June 20, 2008
Revised Manuscript: December 3, 2008
Manuscript Accepted: December 4, 2008
Published: January 14, 2009
Virtual Issues
Vol. 4, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Pierre Blandin, Sandrine Lévêque-Fort, Sandrine Lécart, Jack C. Cossec, Marie-Claude Potier, Zsolt Lenkei, Frédéric Druon, and Patrick Georges, "Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source," Appl. Opt. 48, 553-559 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-3-553
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