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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 31 — Nov. 1, 2009
  • pp: 6050–6058

Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy

Hans Blom and Gunnar Björk  »View Author Affiliations

Applied Optics, Vol. 48, Issue 31, pp. 6050-6058 (2009)

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The theory of autocorrelation-function evaluation in fluorescence correlation spectroscopy is applied to a Lorentzian intensity distribution. An analytical solution to the autocorrelation function for diffusion is deduced for this spatial distribution. Experimental investigation of the distribution is performed using an enlarged detector aperture in a standard confocal setup. The data from the experiment are fitted to the derived autocorrelation function, and a reasonable estimate of the spatial distribution is provided. Estimates are also compared to values computed by molecular detection efficiency simulation. The use of Lorentzian intensity distributions complements conditions where a Gaussian intensity distribution applies, expanding the applicability range of analytical correlation functions.

© 2009 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 22, 2009
Revised Manuscript: September 25, 2009
Manuscript Accepted: October 8, 2009
Published: October 28, 2009

Virtual Issues
Vol. 4, Iss. 13 Virtual Journal for Biomedical Optics

Hans Blom and Gunnar Björk, "Lorentzian spatial intensity distribution in one-photon fluorescence correlation spectroscopy," Appl. Opt. 48, 6050-6058 (2009)

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