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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 6 — Feb. 20, 2009
  • pp: 1042–1046

Real-time imaging of autofluorescence NAD(P)H in single human neutrophils

Leiting Pan, Xinzheng Zhang, Kun Song, Baiquan Tang, Wei Cai, Xian Wu, Romano A. Rupp, and Jingjun Xu  »View Author Affiliations


Applied Optics, Vol. 48, Issue 6, pp. 1042-1046 (2009)
http://dx.doi.org/10.1364/AO.48.001042


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Abstract

Real-time detection of NAD(P)H is particularly important for understanding physiological activities of neutrophils. We scrutinize the performance of weak light detection systems with electron multiplying CCDs (EMCCDs) with regard to the feasibility of valid investigations by autofluorescence NAD(P)H in single human neutrophils. The low-noise amplification facility of EMCCDs is indeed just adequate to permit detection at an irradiation level where neither quenching nor phototoxic effects occur. For demonstration, a neutrophil respiratory burst was triggered and observed in real time. Our low-intensity detection system fulfills all requirements for real-time investigations at high spatiotemporal resolution in the field of neutrophil physiology and pathology.

© 2009 Optical Society of America

OCIS Codes
(110.2970) Imaging systems : Image detection systems
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(260.2510) Physical optics : Fluorescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 26, 2008
Revised Manuscript: November 20, 2008
Manuscript Accepted: January 27, 2009
Published: February 12, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Leiting Pan, Xinzheng Zhang, Kun Song, Baiquan Tang, Wei Cai, Xian Wu, Romano A. Rupp, and Jingjun Xu, "Real-time imaging of autofluorescence NAD(P)H in single human neutrophils," Appl. Opt. 48, 1042-1046 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-6-1042

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