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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 6 — Feb. 20, 2009
  • pp: 1114–1118

Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system

Goro Terakado, Kouyou Watanabe, and Hiroshi Kano  »View Author Affiliations


Applied Optics, Vol. 48, Issue 6, pp. 1114-1118 (2009)
http://dx.doi.org/10.1364/AO.48.001114


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Abstract

We report on a scanning total internal reflection fluorescence microscope with improved imaging properties. In the illumination system of the developed microscope, radial polarization is employed to obtain the point spread function with a single and circular peak. By using radial polarization, the confocal detection method, which can reduce the background noise of the image, is effectively applicable in the optical system. The observed image of a fluorescent particle with the size of 200 nm reveals the improvement of the imaging properties.

© 2009 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: July 28, 2008
Revised Manuscript: January 27, 2009
Manuscript Accepted: January 30, 2009
Published: February 13, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Goro Terakado, Kouyou Watanabe, and Hiroshi Kano, "Scanning confocal total internal reflection fluorescence microscopy by using radial polarization in the illumination system," Appl. Opt. 48, 1114-1118 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-6-1114

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