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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 48, Iss. 7 — Mar. 1, 2009
  • pp: 1347–1357

Differential interference contrast microscope using photonic crystals for phase imaging and three-dimensional shape reconstruction

Laurent Fabre, Yoshihiko Inoue, Takafumi Aoki, and Shojiro Kawakami  »View Author Affiliations


Applied Optics, Vol. 48, Issue 7, pp. 1347-1357 (2009)
http://dx.doi.org/10.1364/AO.48.001347


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Abstract

We present a differential interference contrast microscope using photonic crystals capable of real-time capture of both phase and amplitude components independently without moving parts. Unlike previous methods using rotating polarizers to discriminate each component, we propose using a special camera equipped with an arrayed polarizer whose instant polarization measurement allows real-time acquisition of the phase gradient information. A two-image algorithm is used to reconstruct the phase two- dimensional distribution of biological samples from the gradient information with a transmission-type microscope. We also talk about deducing a sample’s three-dimensional shape for a reflection-type microscope. The efficiency of the method is demonstrated experimentally.

© 2009 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: August 27, 2008
Revised Manuscript: December 19, 2008
Manuscript Accepted: December 23, 2008
Published: February 24, 2009

Virtual Issues
Vol. 4, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Laurent Fabre, Yoshihiko Inoue, Takafumi Aoki, and Shojiro Kawakami, "Differential interference contrast microscope using photonic crystals for phase imaging and three-dimensional shape reconstruction," Appl. Opt. 48, 1347-1357 (2009)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-48-7-1347

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