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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 1 — Jan. 1, 2010
  • pp: 80–85

Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate

Takuo Akimoto and Mitsuru Yasuda  »View Author Affiliations


Applied Optics, Vol. 49, Issue 1, pp. 80-85 (2010)
http://dx.doi.org/10.1364/AO.49.000080


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Abstract

Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al 2 O 3 was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al 2 O 3 had two peaks and one valley in the range from 0 to 300 nm of Al 2 O 3 thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al 2 O 3 thickness as well, and the maximum reflection was observed near the Al 2 O 3 thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the λ / 4 derived by a simple interference theory.

© 2010 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 20, 2009
Revised Manuscript: November 10, 2009
Manuscript Accepted: November 24, 2009
Published: December 21, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Takuo Akimoto and Mitsuru Yasuda, "Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate," Appl. Opt. 49, 80-85 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-1-80


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References

  1. J. R. Lakowicz, “Radiative decay engineering: biophysical and biomedical applications,” Anal. Biochem. 298, 1-24 (2001). [CrossRef]
  2. J. R. Lakowicz, Y. Shen, S. D'Auria, J. Malicka, J. Fang, Z. Gryczynski, and I. Gryczynski, “Radiative decay engineering: 2. Effect of silver island films on fluorescence intensity, lifetime, and resonance energy transfer,” Anal. Biochem. 301, 261-277 (2002). [CrossRef]
  3. J. R. Lakowicz, C. D. Geddes, I. Gryczynski, J. Malicka, Z. Gryczynski, K. Aslan, J. Lukomska, E. Matveeva, J. Zhang, R. Badugu, and J. Huang, “Advances in surface-enhanced fluorescence,” J. Fluoresc. 14, 425-441 (2004). [CrossRef]
  4. P. J. Tarcha, J. Desaja-Gonzalez, S. Rodriguez-Llorente, and R. Aroca, “Surface-enhanced fluorescence on SiO2-coated silver island films,” Appl. Spectrosc. 53, 43-48 (1999). [CrossRef]
  5. J. Malicka, I. Gryczynski, Z. Gryczynski, and J. R. Lakowicz, “Effect of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides,” Anal. Biochem. 315, 57-66 (2003). [CrossRef]
  6. Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006). [CrossRef]
  7. C. R. Sabanayagam and J. R. Lakowicz, “Increasing the sensitivity of DNA microarrays by metal-enhanced fluorescence using surface-bound silver nanoparticles,” Nucleic Acid Res. 35, e13 (2007).
  8. E. G. Matveeva, I. Gryczynski, A. Barnett, Z. Leonenko, J. R. Lakowicz, and Z. Gryczynski, “Metal particle-enhanced fluorescent immunoassay on metal mirrors,” Anal. Biochem. 363, 239-245 (2007). [CrossRef]
  9. Y. Fu and J. R. Lakowicz, “Enhanced fluorescence of Cy5-labeled DNA tethered to silver island films: fluorescence image and time-resolved studies using single-molecule spectroscopy,” Anal. Chem. 78, 6238-6245 (2006). [CrossRef]
  10. W. R. Holland and D. G. Hall, “Waveguide mode enhancement of molecular fluorescence,” Opt. Lett. 10, 414-416 (1985). [CrossRef]
  11. K. G. Sullivan, O. King, C. Sigg, and D. G. Hall, “Directional, enhanced fluorescence from molecular near a periodic surface,” Appl. Opt. 33, 2447-2454 (1994). [CrossRef]
  12. K. G. Sullivan and D. G. Hall, “Enhancement and inhibition of electromagnetic radiation in plane-layered media. I. Plane-wave-spectrum approach to modeling classical effects,” J. Opt. Soc. Am. B 14, 1149-1159 (1997). [CrossRef]
  13. K. G. Sullivan and D. G. Hall, “Enhancement and inhibition of electromagnetic radiation in plane-layered media. II. Enhanced fluorescence in optical waveguide sensors,” J. Opt. Soc. Am. B 14, 1160-1166 (1997). [CrossRef]
  14. E. Le Moal, E. Fort, S. Lévêsque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, “Enhanced fluorescence cell imaging with metal-coated slides,” Biophys. J. 92, 2150-2169 (2007). [CrossRef]
  15. E. Le Moal, E. Fort, S. Lévêsque-Fort, A. Janin, H. Murata, F. P. Cordelières, M. P. Fontaine-Aupart, and C. Ricolleau, “Mirror slides for high-sensitivity cell and tissue fluorescence imaging,” J. Biomed. Opt. 12, 024030 (2007).
  16. T. Akimoto, M. Yasuda, and I. Karube, “Effect of polarization and incident angle of excitation light on the fluorescence enhancement observed with a multilayered substrate fabricated by Ag and Al2O3,” Appl. Opt. 47, 3789-3794 (2008). [CrossRef]
  17. H. Choumane, N. Ha, C. Nelep, A. Chardon, G. O. Reymond, C. Goutel, G. Cerovic, F. Vallet, and C. Weisbuch, “Double interference fluorescence enhancement from reflective slide: application to bicolor microarrays,” Appl. Phys. Lett. 87, 031102 (2005). [CrossRef]

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