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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 1 — Jan. 1, 2010
  • pp: 80–85

Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate

Takuo Akimoto and Mitsuru Yasuda  »View Author Affiliations


Applied Optics, Vol. 49, Issue 1, pp. 80-85 (2010)
http://dx.doi.org/10.1364/AO.49.000080


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Abstract

Fluorescence enhancement from a multilayered substrate fabricated with Ag and Al 2 O 3 was investigated using fluorescein, rhodamine B, Cy3, and Cy5 as fluorophores. The change in the fluorescence enhancement with Al 2 O 3 had two peaks and one valley in the range from 0 to 300 nm of Al 2 O 3 thickness, and such peaks and valley were found to appear periodically. Moreover, the reflection of the excitation light from the multilayered substrate was investigated. The reflection of the excitation light periodically changed depending on the Al 2 O 3 thickness as well, and the maximum reflection was observed near the Al 2 O 3 thickness of the peak fluorescence enhancement. It was found that the periodic changes of the fluorescence enhancement and the reflection of the excitation light could be explained, for the most part, with the integral multiples of the λ / 4 derived by a simple interference theory.

© 2010 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.2520) Medical optics and biotechnology : Fluorescence microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 20, 2009
Revised Manuscript: November 10, 2009
Manuscript Accepted: November 24, 2009
Published: December 21, 2009

Virtual Issues
Vol. 5, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Takuo Akimoto and Mitsuru Yasuda, "Fluorescence enhancement and reflection of the excitation light observed with a multilayered substrate," Appl. Opt. 49, 80-85 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-1-80

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