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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 16 — Jun. 1, 2010
  • pp: 3053–3058

Photoacoustic waves generated in blood studied using pulsed digital holography

Erik Olsson, Per Gren, and Mikael Sjödahl  »View Author Affiliations

Applied Optics, Vol. 49, Issue 16, pp. 3053-3058 (2010)

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We studied photoacoustic waves using pulsed digital holography. The acoustic waves were generated in a reindeer blood target by absorption of an IR laser pulse, λ = 1064 nm and pulse length = 12 ns . The acoustic pressure waves were then imaged in water using a second collimated laser pulse at λ = 532 nm 2 μs after the first IR pulse. Quantitative information on acoustic wave properties such as three-dimensional shape and pressure distribution was calculated by applying the inverse Radon transform on the recorded projection. The pressure pulse had a flat and sharp front parallel with the blood surface, which indicates that the pressure was generated at the blood surface. The generated pressure was proportional to the laser fluence with the proportionality constant equal to 1.8 ± 0.3 cm 1 . According to existing data, the proportionality constant should be 1.4 cm 1 for oxygenated human blood, which made our calculations probable.

© 2010 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.5125) Imaging systems : Photoacoustics

ToC Category:

Original Manuscript: November 11, 2009
Revised Manuscript: March 31, 2010
Manuscript Accepted: April 30, 2010
Published: May 25, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Erik Olsson, Per Gren, and Mikael Sjödahl, "Photoacoustic waves generated in blood studied using pulsed digital holography," Appl. Opt. 49, 3053-3058 (2010)

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  1. E. Lüscher, Photoacoustic Effect: Principles and Applications (Viewg and Sohn, 1984).
  2. O. R. Esenaliev, V. I. Larina, V. K. Larin, J. D. Deyo, M. Motamedi, and S. D. Prough, “Optoacoustic technique for noninvasive monitoring of blood oxygenation: a feasibility study,” Appl. Opt. 41, 4722–4731 (2002). [CrossRef] [PubMed]
  3. R. Kolkman, J. Klaessens, E. Hondebrink, J. Hopman, F. de Mul, W. Steenbergen, J. Thijssen, and T. van Leeuwen, “Photoacoustic determination of blood vessel diameter,” Phys. Med. Biol. 49, 4745–4756 (2004). [CrossRef] [PubMed]
  4. Y. Lao, D. Xing, S. Yang, and L. Xiang, “Noninvasive photoacoustic imaging of the developing vasculature during early tumor growth,” Phys. Med. Biol. 53, 4203–4212 (2008). [CrossRef] [PubMed]
  5. A. P. Gibson, J. C. Hebden, and S. R. Arridge, “Recent advances in diffuse optical imaging,” Phys. Med. Biol. 50, R1–R43 (2005). [CrossRef] [PubMed]
  6. T. Khokhlova, I. Pelivanov, V. Kozhushko, A. Zharinov, V. Solomatin, and A. Karabutov, “Optoacoustic imaging of absorbing objects in a turbid medium: ultimate sensitivity and application to breast cancer diagnostics,” Appl. Opt. 46, 262–272 (2007). [CrossRef] [PubMed]
  7. T. E. Carlsson, R. Mattsson, P. Gren, M. Elfsberg, and J. Tegner, “Combination of schlieren and pulsed TV holography in the study of a high-speed flame jet,” Opt. Lasers Eng. 44, 535–554 (2006). [CrossRef]
  8. R. Mattsson, “Bending and acoustic waves in a water-filled box studied by pulsed TV holography and LDV,” Opt. Lasers Eng. 44, 1146–1157 (2006). [CrossRef]
  9. E. Amer, P. Gren, and M. Sjödahl, “Laser-ablation-induced refractive index fields studied using pulsed digital holographic interferometry,” Opt. Lasers Eng. 47, 793–799 (2009). [CrossRef]
  10. E. Amer, P. Gren, A. Kaplan, and M. Sjödahl, “Impact of an extended source in laser ablation using pulsed digital holographic interferometry and modelling,” Appl. Surf. Sci. 255, 8917–8925 (2009). [CrossRef]
  11. V. E. Gusev and A. A. Karabutov, Laser Optoacoustics(American Institute of Physics, 1993).
  12. M. Takeda, H. Ina, and S. Kobayashi, “Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry,” J. Opt. Soc. Am. 72, 156–160 (1982). [CrossRef]
  13. A. C. Kak and M. Slaney, Principles of Computerized Tomographic Imaging (IEEE, 1988).
  14. H. Eisenberg, “Equation for the refractive index of water,” J. Chem. Phys. 43, 3887–3892 (1965). [CrossRef]
  15. American Institute of Physics Handbook (McGraw-Hill, 1972).
  16. B. T. Cox, J. G. Laufer, and P. C. Beard, “The challenges for quantitative photoacoustic imaging,” Proc. SPIE 7177, 717713 (2009).. [CrossRef]
  17. A. Yaroslasvsky, I. Yaroslasvsky, T. Goldbach, and H. Schwarzmaier, “The optical properties of blood in the near infrared spectral range,” Proc. SPIE 2678, 314–324 (1996). [CrossRef]

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