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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 16 — Jun. 1, 2010
  • pp: 3235–3238

Complex refractive index measurement of biological tissues by attenuated total reflection ellipsometry

Jian-Cheng Lai, Ying-Ying Zhang, Zhen-Hua Li, Hai-Jiao Jiang, and An-Zhi He  »View Author Affiliations

Applied Optics, Vol. 49, Issue 16, pp. 3235-3238 (2010)

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To apply reflection ellipsometry to determine the real and imaginary parts of the refractive index of biological tissues simultaneously, we combine reflection ellipsometry with total internal reflection to warrant minimal influences by the strong scattering and absorption of biological tissues. A K9 glass prism with refractive index 1.51468 at wavelength 632.8 nm and a Glan prism polarizer with an angular sampling interval of 0.1 ° were used in our experimental setup. Using the setup, the complex refractive indices of some typical mammalian tissues were measured under the wavelength of 632.8 nm . The results show that the indices of porcine muscle, liver, pancreas, and dermis tissues were 1.3713 + 0.062 i , 1.3791 + 0.0087 i , 1.3517 + 0.0113 i , and 1.3818 + 0.0049 i , respectively.

© 2010 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3890) Medical optics and biotechnology : Medical optics instrumentation

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: November 30, 2009
Revised Manuscript: March 27, 2010
Manuscript Accepted: April 26, 2010
Published: May 31, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Jian-Cheng Lai, Ying-Ying Zhang, Zhen-Hua Li, Hai-Jiao Jiang, and An-Zhi He, "Complex refractive index measurement of biological tissues by attenuated total reflection ellipsometry," Appl. Opt. 49, 3235-3238 (2010)

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