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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 17 — Jun. 10, 2010
  • pp: 3323–3329

Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy

Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa  »View Author Affiliations


Applied Optics, Vol. 49, Issue 17, pp. 3323-3329 (2010)
http://dx.doi.org/10.1364/AO.49.003323


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Abstract

We present measurements of two-photon excitation (TPE) spectra of various fluorescent proteins with nonlinear Fourier-transform spectroscopy. By using an ultrabroadband laser pulse with a spectrum ranging from 700 to 1100 nm , the absolute TPE spectra of six typical fluorescent proteins (SeBFP, Sapphire, eGFP, eCFP, Venus, DsRed) were measured with high spectral resolution.

© 2010 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(300.6420) Spectroscopy : Spectroscopy, nonlinear

ToC Category:
Spectroscopy

History
Original Manuscript: January 27, 2010
Revised Manuscript: May 7, 2010
Manuscript Accepted: May 14, 2010
Published: June 7, 2010

Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Hiroshi Hashimoto, Keisuke Isobe, Akira Suda, Fumihiko Kannari, Hiroyuki Kawano, Hideaki Mizuno, Atsushi Miyawaki, and Katsumi Midorikawa, "Measurement of two-photon excitation spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy," Appl. Opt. 49, 3323-3329 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-17-3323

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