The absorption and scattering properties of three developmental stages of protozoan parasite Plasmodium falciparum were studied both experimentally and theoretically. Experimentally, the light attenuation and forward scattering from parasites extracted from host erythrocyte cultures were measured with UV–visible spectroscopy. The measured spectra were interpreted theoretically with a model based on the core-shell Mie theory in terms of the structural and compositional characteristics of the protozoa. The model accurately reproduced the features of the measured spectra of all developmental stages. The results show that realistic quantitative estimates of the parasite size, nucleotide, and hemozoin contents can be derived from the UV–visible spectroscopy measurements.
© 2010 Optical Society of America
Original Manuscript: August 25, 2009
Manuscript Accepted: November 24, 2009
Published: January 7, 2010
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics
Yulia M. Serebrennikova, Janus Patel, and Luis H. Garcia-Rubio, "Interpretation of the ultraviolet-visible spectra of malaria parasite Plasmodium falciparum," Appl. Opt. 49, 180-188 (2010)