Short laser pulse technology has significantly contributed to biomedical research, especially via nonlinear optical microscopy. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free, chemical-selective method that is growing in importance as improved methods and light sources develop. Here we discuss different approaches to laser source development for CARS microscopy and highlight the advantages of a multimodal CARS microscope, illustrated by selected applications in biomedical research.
© 2010 Optical Society of America
LASERS: THE FIRST FIFTY YEARS (INVITED ONLY)
Original Manuscript: February 17, 2010
Revised Manuscript: April 4, 2010
Manuscript Accepted: April 26, 2010
Published: June 1, 2010
Adrian F. Pegoraro, Aaron D. Slepkov, Andrew Ridsdale, John Paul Pezacki, and Albert Stolow, "Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy," Appl. Opt. 49, F10-F17 (2010)