OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 26 — Sep. 10, 2010
  • pp: 4890–4897

Ultraviolet analysis of donated corneas: a portable prototype

Victor A. C. Lincoln, Liliane Ventura, and Sidney J. Faria e Sousa  »View Author Affiliations


Applied Optics, Vol. 49, Issue 26, pp. 4890-4897 (2010)
http://dx.doi.org/10.1364/AO.49.004890


View Full Text Article

Enhanced HTML    Acrobat PDF (1081 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

As technology improves human vision, some procedures currently performed may be causing a decrease of the natural UV protection of the cornea. A portable dual beam system prototype was assembled for physicians for clinical studies of these effects on the corneas endowing two types of 300–400 nm evaluations: 1, regularly donated corneas and 2, simulating refractive keratectomy by corneal lamellae removal. The system performs 500 measurements/s, providing ± 0.25 % precision for the transmittance. The measurements performed on the prototype are 95% in agreement with Cary 17 and HR4000CG-UV-NIR Ocean Optics spectrophotometers. Preliminary studies on cadaveric corneas demonstrate that, as the stromal layer is reduced ( 150 μm depth), there is significant loss—an average of 7.1%.—of the cornea’s natural UV protection. The prototype is being tested in an eye bank for routine evaluation of donor corneas.

© 2010 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(300.6540) Spectroscopy : Spectroscopy, ultraviolet
(330.5380) Vision, color, and visual optics : Physiology

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 4, 2010
Revised Manuscript: July 14, 2010
Manuscript Accepted: August 4, 2010
Published: September 3, 2010

Virtual Issues
Vol. 5, Iss. 13 Virtual Journal for Biomedical Optics

Citation
Victor A. C. Lincoln, Liliane Ventura, and Sidney J. Faria e Sousa, "Ultraviolet analysis of donated corneas: a portable prototype," Appl. Opt. 49, 4890-4897 (2010)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-49-26-4890


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. J. Wenk, P. Brenneisen, C. Meewes, M. Wlaschek, T. Peters, R. Blaudschun, W. Ma, L. Kuhr, L. Schneider, and K. Scharffetter-Kochanek, “UV-induced oxidative stress and photoagin,” Curr. Probl. Dermatol. 29, 83–94 (2000). [CrossRef]
  2. L. Kolozsvári, A. Nógrádi, B. Hopp, and Z. Bor, “UV absorbance of the human cornea in the 240- to 400 nmrange,” Invest. Ophthalmol. Visual Sci. 43, 2165–2168(2002).
  3. A. Lembares, X.-H. Hu, and G. W. Kalmus, “Absorption spectra of the córneas in the far ultraviolet region,” Invest. Ophthalmol. Visual Sci. 38, 1283–1287 (1997).
  4. J. S. Hoffman, ed. Assessing the Risks of Trace Gases that can Modify the Stratosphere (Environmental Protection Agency, 1987), EPA 400/1-A 87/001C, Chap. 10.
  5. A. Ringvold, “Corneal epithelium and UV-protection of the eye,” Acta Ophthalmol. 76, 149–153 (1998). [CrossRef]
  6. T. Seiler and P. J. McDonnell, “Excimer laser photorefractive keratectomy,” Surv. Ophthalmol. 40, 89–118 (1995). [CrossRef] [PubMed]
  7. A. W. Chang, A. C. Tsang, J. E. Contreras, P. D. Huynh, C. J. Calvano, T. C. Cmic-Rein, and E. H. Thall, “Corneal tissue ablation depth and the Munnerlyn formula,” J. Cataract Refractive Surg. 29, 1204–1210 (2003). [CrossRef]
  8. L. Ventura, S. J. F. Sousa, A. M. V. Messias, and J. M. Bispo, “System for measuring the transmission spectrum of in vitro corneas,” Physiol. Meas. 21 , 197–207 (2000). [CrossRef] [PubMed]
  9. L. Ventura, G. T. Jesus, G. C. D. Oliveira, and S. J. F. Sousa, “Portable light transmission measuring system for preserved corneas,” Biomed. Eng. Online 4, 70 (2005). [CrossRef] [PubMed]
  10. G. Wollensak, E. Spoerl, M. Wilsch, T. P. Seiler, “Endothelial cell damage after riboflavin—ultraviolet-A treatment in the rabbit,” J. Cataract Refractive Surg. 29, 1786–1790 (2003). [CrossRef]
  11. D. A. Atchison and G. Smith, Optics of the Human Eye (Butterworth–Heinemann, 2000).
  12. J. M. Bland and D. G. Altman, “Statistical methods for assessing agreement between two methods of clinical measurement,” Lancet 327, 307–310 (1986). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited