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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 3 — Jan. 20, 2010
  • pp: 484–491

Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization

Seyoung Moon, Dong Jun Kim, Kyujung Kim, Donghyun Kim, Hosub Lee, Kangtaek Lee, and Seungjoo Haam  »View Author Affiliations

Applied Optics, Vol. 49, Issue 3, pp. 484-491 (2010)

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We have investigated surface-enhanced plasmon resonance detection of DNA hybridization. Surface enhancement was based on the excitation of localized surface plasmon using subwavelength nanogratings, at a 300 nm period, coated with 24-mer ssDNA oligonucleotide, while optical signatures of DNA were amplified at the same time by gold nanoparticles conjugated with complementary ssDNA strands. When using nanoparticles of different sizes, maximum sensitivity enhancement, of more than 18 times, was obtained with nanoparticles of 20 nm diameter. This enhancement is mainly due to nanoparticle- associated signal amplification. Additional surface enhancement boosted the detection sensitivity by 57%. We have also confirmed the sensitivity enhancement to be linearly related to nanoparticle volume.

© 2010 Optical Society of America

OCIS Codes
(240.6680) Optics at surfaces : Surface plasmons
(280.4788) Remote sensing and sensors : Optical sensing and sensors
(050.6624) Diffraction and gratings : Subwavelength structures

ToC Category:
Optics at Surfaces

Original Manuscript: October 22, 2009
Revised Manuscript: December 13, 2009
Manuscript Accepted: December 14, 2009
Published: January 15, 2010

Virtual Issues
Vol. 5, Iss. 3 Virtual Journal for Biomedical Optics

Seyoung Moon, Dong Jun Kim, Kyujung Kim, Donghyun Kim, Hosub Lee, Kangtaek Lee, and Seungjoo Haam, "Surface-enhanced plasmon resonance detection of nanoparticle-conjugated DNA hybridization," Appl. Opt. 49, 484-491 (2010)

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