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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 30 — Oct. 20, 2010
  • pp: 5686–5696

In vivo rat brain measurements of changes in signal intensity depth profiles as a function of temperature using wide-field optical coherence tomography

Manabu Sato, Daisuke Nomura, Takashi Tsunenari, and Izumi Nishidate  »View Author Affiliations

Applied Optics, Vol. 49, Issue 30, pp. 5686-5696 (2010)

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In our previous study, we used optical coherence tomography (OCT) and reported an increase in signal intensity of depth profiles between euthanasia injection and cardiac arrest (CA), demonstrating the potential as a tool for monitoring/diagnosing brain tissue viability [ Appl. Opt. 48, 4354 (2009)]. Here, for the first time to our knowledge, we measured three-dimensional (3D) OCT images through a thinned skull changing temperatures in the rat brain. The measurements were made at 10 min intervals for 210 min to evaluate correlations of temperature with heart rate and ratios of signal intensity (RSI). The 3D image area was 4 m m × 4 m m × 2.8 m m . When the temperature was decreased from 28 ° C to 18 ° C to reduce tissue viability, the heart rate was found to decrease with an increase in RSI. Negative correlation coefficients (CCs) between temperatures and RSIs, and between heart rate and RSIs, were obtained. This indicates that OCT signals increase with reductions of viability caused by decreases in heart rates and temperatures in tissues. These observations correspond to estimations obtained by multiwavelength diffuse reflectance spectroscopy [ Appl. Opt. 47, 4164 (2008)]. CCs and stationary RSIs would depend upon measured positions in tissues. Without injections for euthanasia, a similar rapid increase in RSI has also been measured before CA.

© 2010 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 26, 2010
Revised Manuscript: July 12, 2010
Manuscript Accepted: August 23, 2010
Published: October 12, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Manabu Sato, Daisuke Nomura, Takashi Tsunenari, and Izumi Nishidate, "In vivo rat brain measurements of changes in signal intensity depth profiles as a function of temperature using wide-field optical coherence tomography," Appl. Opt. 49, 5686-5696 (2010)

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