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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 34 — Dec. 1, 2010
  • pp: 6570–6576

Proximity projection grating structured light illumination microscopy

Chung W. See, Chin-Jung Chuang, Shugang Liu, and Michael G. Somekh  »View Author Affiliations

Applied Optics, Vol. 49, Issue 34, pp. 6570-6576 (2010)

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Structured illumination has been employed in fluorescence microscopy to extend its lateral resolution. It has been demonstrated that a factor of 2 improvement can be achieved. In this paper, we introduce a novel optical arrangement that can further improve the resolution. It makes use of a fine grating held in close proximity to the sample. The fringe pattern thus projected onto the sample contains grating vectors substantially higher than those that are possible with the conventional structured illumination setup. We will present experimental results to demonstrate the principle of the technique, and will show that, theoretically, it can achieve an imaging NA approaching 4.

© 2010 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution
(050.6624) Diffraction and gratings : Subwavelength structures

ToC Category:

Original Manuscript: May 21, 2010
Revised Manuscript: July 30, 2010
Manuscript Accepted: August 11, 2010
Published: November 24, 2010

Chung W. See, Chin-Jung Chuang, Shugang Liu, and Michael G. Somekh, "Proximity projection grating structured light illumination microscopy," Appl. Opt. 49, 6570-6576 (2010)

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