Abstract

We investigate photophysical processes that take place in macromolecules of a fluorescent protein mRFP1 under UV excitation [when the energy transfer in a localized donor–acceptor (LDA) pair, which is presented in the molecules of the protein, becomes apparent]. We used a special approach based on the fluorescence laser spectroscopy technique. The energy transfer rates in LDA pairs and photophysical parameters of fluorophores (chromophores) of three spectral forms, which coexist in the ensemble of the macromolecules of this protein, were determined under pulse UV laser excitation.

© 2010 Optical Society of America

Quantitative FRET measurement by high-speed fluorescence excitation and emission spectrometer

Jing Yuan, Leilei Peng, Brett E. Bouma, and Guillermo J. Tearney
Opt. Express 18(18) 18839-18851 (2010)

Pulse-shaping based two-photon FRET stoichiometry

Daniel C. Flynn, Amar R. Bhagwat, Meredith H. Brenner, Marcos F. Núñez, Briana E. Mork, Dawen Cai, Joel A. Swanson, and Jennifer P. Ogilvie
Opt. Express 23(3) 3353-3372 (2015)

Towards in vivo imaging of intramolecular fluorescence resonance energy transfer parameters

Vaibhav Gaind, Kevin J. Webb, Sumith Kularatne, and Charles A. Bouman
J. Opt. Soc. Am. A 26(8) 1805-1813 (2009)

Quantitative multi-color FRET measurements by Fourier lifetime excitation-emission matrix spectroscopy

Ming Zhao, Run Huang, and Leilei Peng
Opt. Express 20(24) 26806-26827 (2012)

Fluorescence lifetime endoscopy using TCSPC for the measurement of FRET in live cells

Gilbert O. Fruhwirth, Simon Ameer-Beg, Richard Cook, Timothy Watson, Tony Ng, and Frederic Festy
Opt. Express 18(11) 11148-11158 (2010)