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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 4 — Feb. 1, 2010
  • pp: 663–667

Optical fiber laser induced fluorescence spectroscopy as a citrus canker diagnostic

E. C. Lins, J. Belasque, Jr., and L. G. Marcassa  »View Author Affiliations

Applied Optics, Vol. 49, Issue 4, pp. 663-667 (2010)

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Citrus canker is a serious disease caused by Xanthomonas citri subsp. citri bacteria, which infects citrus plants (Citrus spp.) leading to large economic losses in citrus production worldwide. In this work, laser induced fluorescence spectroscopy (LIF) was investigated as a diagnostic technique for citrus canker disease in citrus trees at an orchard using a portable optical fiber based spectrometer. For comparison we have applied LIF to leaves contaminated with citrus canker, citrus scab, citrus variegates chlorosis, and Huanglongbing (HLB, Greening). In order to reduce the noise in the data, we collected spectra from ten leaves with visual symptoms of diseases and from five healthy leaves per plant. This procedure is carried out in order to minimize the environmental effect on the spectrum (water and nutrient supply) of each plant. Our results show that this method presents a high sensitivity ( 90 % ), however it does present a low specificity ( 70 % ) for citrus canker diagnostic. We believe that such poor performance is due to the fact that the optical fiber collects light from only a small part of the leaf. Such results may be improved using the fluorescence imaging technique on the whole leaf.

© 2010 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(300.2530) Spectroscopy : Fluorescence, laser-induced
(280.1415) Remote sensing and sensors : Biological sensing and sensors

ToC Category:

Original Manuscript: May 18, 2009
Revised Manuscript: September 21, 2009
Manuscript Accepted: September 25, 2009
Published: January 26, 2010

Virtual Issues
Vol. 5, Iss. 4 Virtual Journal for Biomedical Optics

E. C. Lins, J. Belasque, Jr., and L. G. Marcassa, "Optical fiber laser induced fluorescence spectroscopy as a citrus canker diagnostic," Appl. Opt. 49, 663-667 (2010)

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