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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 49, Iss. 9 — Mar. 20, 2010
  • pp: 1623–1629

Accommodating brightness and exposure levels in densitometry of stained polyacrylamide electrophoresis gels

Han Yen Tan, Tuck Wah Ng, and Oi Wah Liew  »View Author Affiliations

Applied Optics, Vol. 49, Issue 9, pp. 1623-1629 (2010)

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Flatbed scanner densitometers can be operated under various illumination and recording exposure levels. In this work, we show that optical density measurement accuracy, sensitivity, and stability of stained polyacrylamide electrophoresis gel densitometry are crucially dependent on these two factors (brightness and exposure level), notwithstanding that the source is monochromatic, spatially uniform, and the measurements are made using an accurately calibrated step wedge in tandem. We further outline a method to accommodate the intensity deviations over a range of illumination and exposure levels in order to maintain sensitivity and repeatability in the computed optical densities. Comparisons were also made with results from a commercial densitometer.

© 2010 Optical Society of America

OCIS Codes
(110.0110) Imaging systems : Imaging systems
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: November 9, 2009
Revised Manuscript: January 14, 2010
Manuscript Accepted: January 27, 2010
Published: March 15, 2010

Virtual Issues
Vol. 5, Iss. 7 Virtual Journal for Biomedical Optics

Han Yen Tan, Tuck Wah Ng, and Oi Wah Liew, "Accommodating brightness and exposure levels in densitometry of stained polyacrylamide electrophoresis gels," Appl. Opt. 49, 1623-1629 (2010)

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