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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 10 — Apr. 1, 2011
  • pp: 1469–1476

Long-distance axial trapping with Laguerre–Gaussian beams

Raktim Dasgupta, Ravi Shanker Verma, Sunita Ahlawat, Deepa Chaturvedi, and Pradeep Kumar Gupta  »View Author Affiliations

Applied Optics, Vol. 50, Issue 10, pp. 1469-1476 (2011)

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We show that the axial spread of the focal volume of a tightly focused beam propagating through a glass–water interface is much reduced for Laguerre–Gaussian (LG) modes as compared to the TEM 00 mode. Therefore, use of the LG beam helps in achieving a significant improvement of the axial trapping range in optical tweezers. We demonstrate the use of LG modes to manipulate biological cells from the bottom layer of the medium to the top surface layer. Exposure of the cells to a higher oxygen concentration at the surface layer is used for estimation of the intramembrane oxygen diffusion rate.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Tweezers or Optical Manipulation

Original Manuscript: November 15, 2010
Revised Manuscript: February 12, 2011
Manuscript Accepted: February 13, 2011
Published: March 31, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Raktim Dasgupta, Ravi Shanker Verma, Sunita Ahlawat, Deepa Chaturvedi, and Pradeep Kumar Gupta, "Long-distance axial trapping with Laguerre–Gaussian beams," Appl. Opt. 50, 1469-1476 (2011)

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