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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 13 — May. 1, 2011
  • pp: 1839–1842

Implementation of vibrational phase contrast coherent anti-Stokes Raman scattering microscopy

Martin Jurna, Jennifer L. Herek, and Herman L. Offerhaus  »View Author Affiliations


Applied Optics, Vol. 50, Issue 13, pp. 1839-1842 (2011)
http://dx.doi.org/10.1364/AO.50.001839


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Abstract

Detection of molecules using vibrational resonances in the fingerprint region for narrowband coherent anti-Stokes Raman scattering (CARS) is challenging. The spectrum is highly congested resulting in a large background and a reduced specificity. Recently we introduced vibrational phase contrast CARS (VPC-CARS) microscopy as a technique capable of detecting both the amplitude and phase of the CARS signal, providing background-free images and high specificity. In this paper we present a new implementation of VPC-CARS based on a third-order cascaded phase-preserving chain, where the CARS signal is generated at a single (constant) wavelength independent of the vibrational frequency that is addressed. This implementation will simplify the detection side considerably.

© 2011 Optical Society of America

OCIS Codes
(190.4410) Nonlinear optics : Nonlinear optics, parametric processes
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(300.6310) Spectroscopy : Spectroscopy, heterodyne

ToC Category:
Spectroscopy

History
Original Manuscript: January 4, 2011
Revised Manuscript: March 17, 2011
Manuscript Accepted: March 17, 2011
Published: April 22, 2011

Virtual Issues
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Martin Jurna, Jennifer L. Herek, and Herman L. Offerhaus, "Implementation of vibrational phase contrast coherent anti-Stokes Raman scattering microscopy," Appl. Opt. 50, 1839-1842 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-13-1839

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