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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 16 — Jun. 1, 2011
  • pp: 2376–2382

Fast-updating and nonrepeating Lissajous image reconstruction method for capturing increased dynamic information

Christopher L. Hoy, Nicholas J. Durr, and Adela Ben-Yakar  »View Author Affiliations

Applied Optics, Vol. 50, Issue 16, pp. 2376-2382 (2011)

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We present a fast-updating Lissajous image reconstruction methodology that uses an increased image frame rate beyond the pattern repeat rate generally used in conventional Lissajous image reconstruction methods. The fast display rate provides increased dynamic information and reduced motion blur, as compared to conventional Lissajous reconstruction, at the cost of single-frame pixel density. Importantly, this method does not discard any information from the conventional Lissajous image reconstruction, and frames from the complete Lissajous pattern can be displayed simultaneously. We present the theoretical background for this image reconstruction methodology along with images and video taken using the algorithm in a custom-built miniaturized multiphoton microscopy system.

© 2011 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.3010) Image processing : Image reconstruction techniques
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Image Processing

Original Manuscript: November 29, 2010
Revised Manuscript: February 7, 2011
Manuscript Accepted: February 8, 2011
Published: May 23, 2011

Virtual Issues
Vol. 6, Iss. 7 Virtual Journal for Biomedical Optics

Christopher L. Hoy, Nicholas J. Durr, and Adela Ben-Yakar, "Fast-updating and nonrepeating Lissajous image reconstruction method for capturing increased dynamic information," Appl. Opt. 50, 2376-2382 (2011)

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  1. D. L. Dickensheets and G. S. Kino, “Micromachined scanning confocal optical microscope,” Opt. Lett. 21, 764–766(1996). [CrossRef] [PubMed]
  2. F. Helmchen, M. S. Fee, D. W. Tank, and W. Denk, “A miniature head-mounted two-photon microscope: high-resolution brain imaging in freely moving animals,” Neuron 31, 903–912(2001). [CrossRef] [PubMed]
  3. B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, “In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microendoscope,” Opt. Lett. 30, 2272–2274 (2005). [CrossRef] [PubMed]
  4. C. L. Hoy, N. J. Durr, P. Chen, W. Piyawattanametha, H. Ra, O. Solgaard, and A. Ben-Yakar, “Miniaturized probe for femtosecond laser microsurgery and two-photon imaging,” Opt. Express 16, 9996–10005 (2008). [CrossRef] [PubMed]
  5. T.-M. Liu, M.-C. Chan, I. H. Chen, S.-H. Chia, and C.-K. Sun, “Miniaturized multiphoton microscope with a 24 Hz frame-rate,” Opt. Express 16, 10501–10506 (2008). [CrossRef] [PubMed]
  6. J. A. Lissajous, “Note sur un nouveau moyen de mettre en évidence le mouvement vibratoire des corps,” C. R. Hebd. Seances Acad. Sci. 41, 93–95 (1855).
  7. J. T. C. Liu, M. J. Mandella, N. O. Loewke, H. Haeberle, H. Ra, W. Piyawattanametha, O. Solgaard, G. S. Kino, and C. H. Contag, “Micromirror-scanned dual-axis confocal microscope utilizing a gradient-index relay lens for image guidance during brain surgery,” J. Biomed. Opt. 15, 026029 (2010). [CrossRef] [PubMed]
  8. C. L. Hoy, W. Piyawattabanetha, H. Ra, O. Solgaard, and A. Ben-Yakar, “Optical design and imaging performance testing of a 9.6 mm diameter femtosecond laser microsurgery probe,” Opt. Express 19, 10536–10552 (2011). [PubMed]
  9. C. Landis, “Determinants of the critical flicker-fusion threshold,” Physiol. Rev. 34, 259–286 (1954). [PubMed]

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