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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 19 — Jul. 1, 2011
  • pp: 3311–3315

Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy

Tze-Hsuan Liu, Jian-Long Xiao, Chau-Hwang Lee, and Jiunn-Yuan Lin  »View Author Affiliations

Applied Optics, Vol. 50, Issue 19, pp. 3311-3315 (2011)

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We use optical tweezers to trap a unilamellar phospholipid vesicle and measure the out-of-plane thermal fluctuations by using differential confocal microscopy. Bending moduli of the lipid membranes are calculated directly from the mean-square values of the fluctuation amplitudes. Owing to the refractive index contrast between the inner and outer solutions of the vesicle, optical tweezers trap the vesicle laterally and improve the reliability of the measured fluctuation amplitudes along the optical axis. Bending moduli of membranes in gel or fluid phases obtained by the combination of differential confocal microscopy and optical tweezers are close to those reported previously. We also obtain the bending modulus of sphingomyelin membranes in the gel phase, which was not reported previously.

© 2011 Optical Society of America

OCIS Codes
(140.7010) Lasers and laser optics : Laser trapping
(170.1790) Medical optics and biotechnology : Confocal microscopy

ToC Category:
Lasers and Laser Optics

Original Manuscript: April 14, 2011
Revised Manuscript: May 15, 2011
Manuscript Accepted: May 16, 2011
Published: July 1, 2011

Virtual Issues
Vol. 6, Iss. 8 Virtual Journal for Biomedical Optics

Tze-Hsuan Liu, Jian-Long Xiao, Chau-Hwang Lee, and Jiunn-Yuan Lin, "Measurement of membrane rigidity on trapped unilamellar phospholipid vesicles by using differential confocal microscopy," Appl. Opt. 50, 3311-3315 (2011)

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