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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 28 — Oct. 1, 2011
  • pp: 5361–5368

Beam position stabilization for a confocal multiphoton microscope

P. Groß, L. Kleinschmidt, S. Beer, and C. Fallnich  »View Author Affiliations


Applied Optics, Vol. 50, Issue 28, pp. 5361-5368 (2011)
http://dx.doi.org/10.1364/AO.50.005361


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Abstract

The influence of beam-pointing on scanning confocal microscopy is investigated. The beam displacement is measured using a quadrant photodiode, and the apparent movement of a sub-micron-sized particle observed by second-harmonic microscopy is linked to the beam displacement. A simple beam-pointing stabilization is implemented, and improvement of beam stability by three orders of magnitude on long time scales is achieved.

© 2011 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.5810) Microscopy : Scanning microscopy
(140.3425) Lasers and laser optics : Laser stabilization

ToC Category:
Microscopy

History
Original Manuscript: June 14, 2011
Revised Manuscript: July 29, 2011
Manuscript Accepted: July 30, 2011
Published: September 21, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Citation
P. Groß, L. Kleinschmidt, S. Beer, and C. Fallnich, "Beam position stabilization for a confocal multiphoton microscope," Appl. Opt. 50, 5361-5368 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-28-5361

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