The influence of beam-pointing on scanning confocal microscopy is investigated. The beam displacement is measured using a quadrant photodiode, and the apparent movement of a sub-micron-sized particle observed by second-harmonic microscopy is linked to the beam displacement. A simple beam-pointing stabilization is implemented, and improvement of beam stability by three orders of magnitude on long time scales is achieved.
© 2011 Optical Society of America
Original Manuscript: June 14, 2011
Revised Manuscript: July 29, 2011
Manuscript Accepted: July 30, 2011
Published: September 21, 2011
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics
P. Groß, L. Kleinschmidt, S. Beer, and C. Fallnich, "Beam position stabilization for a confocal multiphoton microscope," Appl. Opt. 50, 5361-5368 (2011)