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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 34 — Dec. 1, 2011
  • pp: 6336–6342

Thermal diffusivity imaging with the thermal lens microscope

Oluwatosin O. Dada, Peter E. Feist, and Norman J. Dovichi  »View Author Affiliations


Applied Optics, Vol. 50, Issue 34, pp. 6336-6342 (2011)
http://dx.doi.org/10.1364/AO.50.006336


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Abstract

A coaxial thermal lens microscope was used to generate images based on both the absorbance and thermal diffusivity of histological samples. A pump beam was modulated at frequencies ranging from 50 kHz to 5 MHz using an acousto-optic modulator. The pump and a CW probe beam were combined with a dichroic mirror, directed into an inverted microscope, and focused onto the specimen. The change in the transmitted probe beam’s center intensity was detected with a photodiode. The photodiode’s signal and a reference signal from the modulator were sent to a high-speed lock-in amplifier. The in-phase and quadrature signals were recorded as a sample was translated through the focused beams and used to generate images based on the amplitude and phase of the lock-in amplifier’s signal. The amplitude is related to the absorbance and the phase is related to the thermal diffusivity of the sample. Thin sections of stained liver and bone tissues were imaged; the contrast and signal-to-noise ratio of the phase image was highest at frequencies from 0.1 1 MHz and dropped at higher frequencies. The spatial resolution was 2.5 μm for both amplitude and phase images, limited by the pump beam spot size.

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.0180) Microscopy : Microscopy
(190.4870) Nonlinear optics : Photothermal effects

ToC Category:
Microscopy

History
Original Manuscript: July 25, 2011
Revised Manuscript: August 26, 2011
Manuscript Accepted: August 28, 2011
Published: November 22, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Oluwatosin O. Dada, Peter E. Feist, and Norman J. Dovichi, "Thermal diffusivity imaging with the thermal lens microscope," Appl. Opt. 50, 6336-6342 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-34-6336

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