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Dual-medium quantitative measurement simulation on cells |
Applied Optics, Vol. 50, Issue 35, pp. 6440-6445 (2011)
http://dx.doi.org/10.1364/AO.50.006440
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Abstract
For research on inhomogeneous cells, we present a simulation method called the dual-medium quantitative (DMQ) measurement simulation method, which is realized by combining phase-shifting digital holography with DMQ analysis. The reliability of this method is confirmed by comparing the simulated phase map with the experimental one by the Hilbert phase microscope [
J. Phys. Chem. A
113, 13327 (2009)], and its ability for studying inhomogeneous cells is demonstrated with measurements of a simulated HeLa cell. The average deviation and the relative deviation of physical thickness and axially averaged refractive index are
© 2011 Optical Society of America
OCIS Codes
(120.2880) Instrumentation, measurement, and metrology : Holographic interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(120.6650) Instrumentation, measurement, and metrology : Surface measurements, figure
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(090.1995) Holography : Digital holography
ToC Category:
Holography
History
Original Manuscript: June 2, 2011
Revised Manuscript: August 15, 2011
Manuscript Accepted: August 16, 2011
Published: December 2, 2011
Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Yawei Wang, Weifeng Jin, and Naifei Ren, "Dual-medium quantitative measurement simulation on cells," Appl. Opt. 50, 6440-6445 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-35-6440
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