Optical projection tomography (OPT) is a noninvasive imaging technique that enables imaging of small specimens ( $<1\mathrm{cm}$ ), such as organs or animals in early developmental stages. In this paper, we present a set of computational methods that can be applied to the acquired data sets in order to correct for (a) unknown background or illumination intensity distributions over the field of view, (b) intensity spikes in single CCD pixels (so-called “hot pixels”), and (c) refractive index mismatch between the media in which the specimens are embedded and the environment. We have tested these correction methods using a variety of samples and present results obtained from Parhyale hawaiensis embedded in glycerol and in sea water. Successful reconstructions of fluorescence and absorption OPT images have been obtained for weakly scattering specimens embedded in media with nonmatched refractive index, thus advancing OPT toward routine in vivo imaging.

© 2011 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Multimedia

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Backpropagation in turbid media (JOSAA)
• Three-Dimensional Optical Tomographic Imaging of Supersonic Jets through Inversion of Phase Data Obtained through the Transport-of-Intensity Equation (AO)
• Hard-x-ray region tomographic reconstruction of the refractive-index gradient vector field: imaging principles and comparisons with diffraction-enhanced-imaging-based computed tomography (OL)
• Quantitative flow visualization in supersonic jets through tomographic inversion of wavefronts estimated through shadow casting (AO)
• An adaptive smoothness regularization algorithm for optical tomography (OE)

Related Conference Papers

• Suppression of scattering effect using spatially dependent point spread function
• Imaging of absorption distribution in diffuse medium using backscattered light
• Tomographic imaging of biological tissue by time-resolved, model-based, iterative image reconstruction
• Three-dimensional optical image reconstruction: finite element approach
• Inverse reconstruction of three-dimensional tomographic images of objects in turbid media from time-sliced two-dimensional transmission measurements