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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 50, Iss. 4 — Feb. 1, 2011
  • pp: 392–398

Improved reconstructions and generalized filtered back projection for optical projection tomography

Udo Jochen Birk, Alex Darrell, Nikos Konstantinides, Ana Sarasa-Renedo, and Jorge Ripoll  »View Author Affiliations

Applied Optics, Vol. 50, Issue 4, pp. 392-398 (2011)

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Optical projection tomography (OPT) is a noninvasive imaging technique that enables imaging of small specimens ( < 1 cm ), such as organs or animals in early developmental stages. In this paper, we present a set of computational methods that can be applied to the acquired data sets in order to correct for (a) unknown background or illumination intensity distributions over the field of view, (b) intensity spikes in single CCD pixels (so-called “hot pixels”), and (c) refractive index mismatch between the media in which the specimens are embedded and the environment. We have tested these correction methods using a variety of samples and present results obtained from Parhyale hawaiensis embedded in glycerol and in sea water. Successful reconstructions of fluorescence and absorption OPT images have been obtained for weakly scattering specimens embedded in media with nonmatched refractive index, thus advancing OPT toward routine in vivo imaging.

© 2011 Optical Society of America

OCIS Codes
(100.6950) Image processing : Tomographic image processing
(170.3010) Medical optics and biotechnology : Image reconstruction techniques
(170.6960) Medical optics and biotechnology : Tomography
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Image Processing

Original Manuscript: June 10, 2010
Revised Manuscript: November 27, 2010
Manuscript Accepted: December 13, 2010
Published: January 26, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Udo Jochen Birk, Alex Darrell, Nikos Konstantinides, Ana Sarasa-Renedo, and Jorge Ripoll, "Improved reconstructions and generalized filtered back projection for optical projection tomography," Appl. Opt. 50, 392-398 (2011)

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