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Effect of growth media and washing on the spectral signatures of aerosolized biological simulants |
Applied Optics, Vol. 50, Issue 6, pp. 788-796 (2011)
http://dx.doi.org/10.1364/AO.50.000788
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Abstract
We have evaluated the influence of growth media and washing on the laser-induced fluorescence spectra of bacteria. Three different bacterial simulants were cultured in three types of growth media. Three kinds of samples were generated from each culture: the culture itself, the growth medium alone, and a triple-washed sample. The materials were injected as aerosols in a lab-sized lidar aerosol chamber to obtain their spectra. Using two different analysis approaches, signature variations were observed between the three kinds of samples for most combinations of growth media/bacteria. This study concludes that the culture media used influences the spectral signatures.
OCIS Codes
(260.7190) Physical optics : Ultraviolet
(280.1100) Remote sensing and sensors : Aerosol detection
(280.3640) Remote sensing and sensors : Lidar
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6170) Spectroscopy : Spectra
(280.1415) Remote sensing and sensors : Biological sensing and sensors
ToC Category:
Remote Sensing and Sensors
History
Original Manuscript: October 7, 2010
Manuscript Accepted: November 18, 2010
Published: February 10, 2011
Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Christian Laflamme, Jean-Robert Simard, Sylvie Buteau, Pierre Lahaie, Denis Nadeau, Bernard Déry, Olivier Houle, Pierre Mathieu, Gilles Roy, Jim Ho, and Caroline Duchaine, "Effect of growth media and washing on the spectral signatures of aerosolized biological simulants," Appl. Opt. 50, 788-796 (2011)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-50-6-788
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