Attenuated total reflectance Fourier transform infrared spectroscopy is used to monitor the adsorption of 100 nm 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) phospholipid vesicles to the surfaces of Ge, electrolessly deposited Au, and a well formed self-assembled monolayer of 1-octadecanethiol. The interaction of DPPC vesicles in solution with these different surfaces yields distinctly different surface structures: intact DPPC vesicles on Ge, a supported phospholipid bilayer on an electrolessly deposited Au surface, and a phospholipid monolayer onto the hydrophobic self-assembled monolayer. IR peak position, bandwidth, and intensity are used to confirm structure formation and quantitation of the amount of lipid that desorbs during film formation.
© 2012 Optical Society of America
Original Manuscript: January 27, 2012
Revised Manuscript: February 15, 2012
Manuscript Accepted: March 2, 2012
Published: May 15, 2012
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics
Mateo R. Hernandez, Elyse N. Towns, Terry C. Ng, Brian C. Walsh, Richard Osibanjo, Atul N. Parikh, and Donald P. Land, "Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures," Appl. Opt. 51, 2842-2846 (2012)