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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 51, Iss. 23 — Aug. 10, 2012
  • pp: 5705–5712

Measurement of the trapping efficiency of an elliptical optical trap with rigid and elastic objects

Antti Kauppila, Matti Kinnunen, Artashes Karmenyan, and Risto Myllylä  »View Author Affiliations


Applied Optics, Vol. 51, Issue 23, pp. 5705-5712 (2012)
http://dx.doi.org/10.1364/AO.51.005705


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Abstract

Optical tweezers and their various modifications offer a sophisticated way to perform noncontact cell manipulation. In this paper, we quantify forces existing in an elliptical trap formed by two cylindrical lenses and compare the results with a point optical trap case. The trapping efficiency of point and elliptical traps was analyzed by measuring the Q values of both traps. Polystyrene microspheres and red blood cells (RBCs) were used as samples. Stretching of the RBC was taken into account in the Q value measurements. Although the Q value of a point optical trap is larger than that of an elliptical trap when measured for a single RBC, we can manipulate the orientation of an RBC in a point trap with the elliptical trap and can also trap several RBCs simultaneously in the elliptical trap far from the cuvette surfaces by using a long-working-distance water immersion objective. This opens new possibilities for studying light–matter interactions at the cellular level.

© 2012 Optical Society of America

OCIS Codes
(120.4820) Instrumentation, measurement, and metrology : Optical systems
(350.4855) Other areas of optics : Optical tweezers or optical manipulation

ToC Category:
Optical Tweezers or Optical Manipulation

History
Original Manuscript: April 27, 2012
Revised Manuscript: June 30, 2012
Manuscript Accepted: July 6, 2012
Published: August 8, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Antti Kauppila, Matti Kinnunen, Artashes Karmenyan, and Risto Myllylä, "Measurement of the trapping efficiency of an elliptical optical trap with rigid and elastic objects," Appl. Opt. 51, 5705-5712 (2012)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-51-23-5705


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