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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 51, Iss. 33 — Nov. 20, 2012
  • pp: 8034–8040

Index mismatch aberration correction over long working distances using spatial light modulation

Bergin Gjonaj, Patrick Johnson, Mischa Bonn, and Katrin F. Domke  »View Author Affiliations

Applied Optics, Vol. 51, Issue 33, pp. 8034-8040 (2012)

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For many microscopy applications, millimeters-long free working distances (LWD) are required. However, the high resolution and contrast of LWD objectives operated in air are lost when introducing glass and/or liquid with the sample. We propose to use spatial light modulation to correct for such beam aberrations caused by refractive index mismatches. Focusing a monochromatic laser beam with a 10 mm working distance air objective (50×, 0.5 NA) through air, glass, and water, we manage to restore a sharp, intense focus (FWHM<2λ) by adaptive beam phase shaping. Our approach offers a practical and cost-effective route to high resolution and contrast microscopy using LWD air objectives, extending their usage beyond applications in air.

© 2012 Optical Society of America

OCIS Codes
(220.1000) Optical design and fabrication : Aberration compensation
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

Original Manuscript: August 8, 2012
Revised Manuscript: September 18, 2012
Manuscript Accepted: October 8, 2012
Published: November 20, 2012

Virtual Issues
Vol. 7, Iss. 12 Virtual Journal for Biomedical Optics

Bergin Gjonaj, Patrick Johnson, Mischa Bonn, and Katrin F. Domke, "Index mismatch aberration correction over long working distances using spatial light modulation," Appl. Opt. 51, 8034-8040 (2012)

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