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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 51, Iss. 9 — Mar. 20, 2012
  • pp: 1336–1351

Measurement of light absorption by aquatic particles: improvement of the quantitative filter technique by use of an integrating sphere approach

Rüdiger Röttgers and Steffen Gehnke  »View Author Affiliations


Applied Optics, Vol. 51, Issue 9, pp. 1336-1351 (2012)
http://dx.doi.org/10.1364/AO.51.001336


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Abstract

Determination of particulate absorption in natural waters is often made by measuring the transmittance of samples on glass-fiber filters with the so-called quantitative filter technique (QFT). The accuracy of this technique is limited due to variations in the optical properties of the sample/filter composite, and due to uncertainties in the path-length amplification induced by multiple scattering inside the filter. Some variations in the optical properties of the sample/filter composite can be compensated by additional measurements of the filter’s reflectance (transmittance–reflectance method [T-R] [TassanS.FerrariG. M., Limnol. Oceanogr. 40, 1358 (1995)]). We propose a different, rarely used approach, namely to measure the filter’s absorptance in the center of a large integrating sphere, to avoid problems with light losses due to scattering. A comparison with other QFTs includes a sensitivity study for different error sources and determination of path-length amplification factors for each measurement technique. Measurements with a point-source integrating-cavity absorption meter were therefore used to determine the true absorption. Filter to filter variability induced a much lower error in absorptance compared to a measured transmittance. This reduced error permits more accurate determination of the usually low absorption coefficient in the near IR spectral region. The error of the T-R method was lower than that of the transmittance measurement but slightly higher than that of an absorptance measurement. The mean path-length amplification was much higher for the absorptance measurement compared to the T-R method (4.50 versus 2.45) but was found to be largely independent of wavelength and optical density. With natural samples the path-length amplification was less variable for the absorptance measurement, reducing the overall error for absorption to less than ±14%, compared to ±25% for the T-R method.

© 2012 Optical Society of America

OCIS Codes
(010.4450) Atmospheric and oceanic optics : Oceanic optics
(010.1030) Atmospheric and oceanic optics : Absorption

ToC Category:
Atmospheric and Oceanic Optics

History
Original Manuscript: September 13, 2011
Revised Manuscript: December 2, 2011
Manuscript Accepted: December 8, 2011
Published: March 15, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics
April 30, 2012 Spotlight on Optics

Citation
Rüdiger Röttgers and Steffen Gehnke, "Measurement of light absorption by aquatic particles: improvement of the quantitative filter technique by use of an integrating sphere approach," Appl. Opt. 51, 1336-1351 (2012)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-51-9-1336


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References

  1. K. Shibata, A. A. Benson, and M. Calvin, “The absorption spectra of suspension of living micro-organisms,” Biochim. Biophys. Acta 15, 461–470 (1954). [CrossRef]
  2. K. Shibata, “Spectrophotometry of intact biological materials. absolute and relative measurements of their transmission, reflection, and absorption spectra,” J. Biochem. 45, 599–623 (1958).
  3. C. S. Yentsch, “A non-extractive method for the quantitative estimation of chlorophyll in algal cultures,” Nature 179, 1302–1304 (1957). [CrossRef]
  4. R. M. Pope, A. D. Weidemann, and E. S. Fry, “Integrating cavity absorption meter measurements of dissolved substances and suspended particles in ocean water,” Dyn. Atmos. Oceans 31, 307–320 (2000). [CrossRef]
  5. R. Röttgers, C. Häse, and R. Doerffer, “Determination of particulate absorption of microalgae using a point source integrating cavity absorption meter,” Limnol. Oceanogr. Methods 5, 1–12 (2007). [CrossRef]
  6. B. G. Mitchell, A. Bricaud, K. Carder, J. Cleveland, G. Ferrari, R. Gould, M. Kahru, M. Kishino, H. Maske, T. Moisan, L. Moore, N. Nelson, D. Phinney, R. Reynolds, H. Sosik, D. Stramski, S. Tassan, C. Trees, A. Weidemann, J. Wieland, and A. Vodacek, “Determination of spectral absorption coefficients of particles, dissolved material and phytoplankton for discrete water samples,” in Ocean Optics Protocols for Satellite Ocean Color Sensor Validation, Revision 2, G. S. Fargion and J. L. Mueller, eds., NASA/TM-2000-209966, NASA Goddard Space Flight Center, Greenbelt, Md., 2000, pp. 125–153.
  7. N. B. Nelson and B. B. Prézelin, “Calibration of an integrating sphere for determining the absorption coefficient of scattering suspension,” Appl. Opt. 32, 6710–6717 (1993). [CrossRef]
  8. J. T. O. Kirk, Light and Photosynthesis in Aquatic Ecosystems, 2nd ed. (Cambridge University, 1994).
  9. J. Ducha and S. Kubin, “Measurements of in vivo absorption spectra of microscopic algae using bleached cells as a reference sample,” Arch. Hydrobiol. Suppl. 49, 199–213 (1976).
  10. D. Stramski and J. Piskozub, “Estimation of scattering error in spectrophotometric measurements of light absorption by aquatic particles from three-dimensional radiative transfer simulations,” Appl. Opt. 42, 3634–3646 (2003). [CrossRef]
  11. H. Haardt and H. Maske, “Specific in vivo absorption coefficient of chlorophyll a at 675 nm,” Limnol. Oceanogr. 32, 608–619 (1987). [CrossRef]
  12. M. Babin and D. Stramski, “Light absorption by aquatic particles in the near-infrared spectral region,” Limnol. Oceanogr. 47, 911–915 (2002). [CrossRef]
  13. M. Babin and D. Stramski, “Variations in the mass-specific absorption coefficient of mineral particles suspended in water,” Limnol. Oceanogr. 49, 756–767 (2004). [CrossRef]
  14. D. Stramski, S. B. Wozniak, and P. J. Flatau, “Optical properties of Asian mineral dust suspended in seawater,” Limnol. Oceanogr. 49, 749–755 (2004). [CrossRef]
  15. D. Stramski, M. Babin, and S. Wozniak, “Variations in the optical properties of terrigeneous mineral-rich particulate matter suspended in seawater,” Limnol. Oceanogr. 52, 2418–2433 (2007). [CrossRef]
  16. S. Tassan and G. M. Ferrari, “Variability of light absorption by aquatic particle in the near-infrared spectral region,” Appl. Opt. 42, 4802–4810 (2003). [CrossRef]
  17. C. S. Yentsch, “Measurements of visible light absorption by particulate matter in the ocean,” Limnol. Oceanogr. 7, 207–217 (1962). [CrossRef]
  18. H. G. Trüper and C. S. Yentsch, “Use of glass fiber filters for the rapid preparation of in vivo absorption spectra of photosynthetic bacteria,” J. Bacteriol. 94, 1255–1256(1967).
  19. B. G. Mitchell, “Algorithms for determining the absorption coefficient for aquatic particulates using the quantitative filter technique,” Proc SPIE 1302, 137–148 (1990). [CrossRef]
  20. S. Tassan and G. M. Ferrari, “An alternative approach to absorption measurements of aquatic particles retained on filters,” Limnol. Oceanogr. 40, 1358–1368 (1995). [CrossRef]
  21. S. Tassan and G. M. Ferrari, “A sensitivity analysis of the ‘transmittance-reflectance’ method for measuring light absorption by aquatic particles,” J. Plankton Res. 24, 757–774 (2002). [CrossRef]
  22. W. L. Butler, “Absorption of light by turbid samples,” J. Opt. Soc. Am. 52, 292–299 (1962). [CrossRef]
  23. B. G. Mitchell and D. A. Kiefer, “Chlorophyll a specific absorption and fluorescence excitation spectra for light-limited phytoplankton,” Deep-Sea Res. A 35, 639–663(1988). [CrossRef]
  24. A. Bricaud and D. Stramski, “Spectral absorption coefficients of living phytoplankton and nonalgal biogenous matter: a comparison between the Peru upwelling area and the Sargasso Sea,” Limnol. Oceanogr. 35, 562–582 (1990). [CrossRef]
  25. C. S. Cleveland and A. D. Weidemann, “Quantifying absorption by aquatic particles: a multiple scattering correction for glass-fiber filters,” Limnol. Oceanogr. 38, 1321–1327 (1993). [CrossRef]
  26. B. Arbones, F. G. Figueiras, and M. Zapata, “Determination of phytoplankton absorption coefficient in natural seawater samples: evidence of a unique equation to correct the path-length amplification on glass-fiber filters,” Mar. Ecol. Prog. Ser. 137, 293–304 (1996). [CrossRef]
  27. Z. V. Finkel and A. J. Irwin, “Light absorption by phytoplankton and the filter amplification correction: cell size and species effects,” J. Exp. Mar. Biol. Ecol. 259, 51–61(2001). [CrossRef]
  28. C. S. Roesler, “Theoretical and experimental approaches to improve the accuracy of particulate absorption coefficients derived from the quantitative filter technique,” Limnol. Oceanogr. 43, 1649–1660 (1998). [CrossRef]
  29. J. C. Goldman and M. R. Dennet, “Susceptibility of some marine phytoplankton species to cell breakage during filtration and post-filtration rinsing,” J. Exp. Mar. Biol. Ecol. 86, 47–58 (1985). [CrossRef]
  30. D. Stramski, “Artifacts in measuring absorption spectra of phytoplankton collected on a filter,” Limnol. Oceanogr. 35, 1804–1809 (1990). [CrossRef]
  31. H. M. Sosik, “Storage of marine particulate samples for light-absorption measurements,” Limnol. Oceanogr. 44, 1139–1141 (1999). [CrossRef]
  32. I. Laurion, F. Blouin, and S. Roy, “The quantitative filter technique for measuring phytoplankton absorption: interference by MAAS in the UV waveband,” Limnol. Oceanogr. Methods 1, 1–9 (2003). [CrossRef]
  33. H. Maske and H. Haardt, “Quantitative in vivo absorption spectra of phytoplankton: detrital absorption and comparison with fluorescence excitation spectra,” Limnol. Oceanogr. 32, 620–633 (1987). [CrossRef]
  34. S. G. H. Simis, S. W. M. Peters, and H. J. Gons, “Remote sensing of the cyanobacterial pigment phycocyanin in turbid inland water,” Limnol. Oceanogr. 50, 237–245(2005). [CrossRef]
  35. F. M. P. Saldanha-Correa, S. M. F. Gianesella, and J. J. Barrera-Alba, “A comparison of the retention capability among three different glass-fiber filters used for chlorophyll-a determinations,” Braz. J. Oceanogr. 52, 243–247 (2004). [CrossRef]
  36. R. Röttgers and R. Doerffer, “Measurements of optical absorption by chromophoric dissolved organic matter using a point-source integrating-cavity absorption meter,” Limnol. Oceanogr. Methods 5, 126–135 (2007). [CrossRef]
  37. S. Tassan and G. M. Ferrari, “Measurement of light absorption by aquatic particles retained on filters: determination of the optical path-length amplification by the ‘transmittance-reflectance’ method,” J. Plankton Res. 20, 1699–1709 (1998). [CrossRef]
  38. S. E. Lohrenz, “A novel theoretical approach to correct for path-length amplification and variable sampling loading in measurements of particulate spectral absorption by the quantitative filter technique,” J. Plankton Res. 22, 639–657 (2000). [CrossRef]

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