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Quantitative phase microscopy using dual-plane in-line digital holography |
Applied Optics, Vol. 51, Issue 9, pp. 1387-1395 (2012)
http://dx.doi.org/10.1364/AO.51.001387
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Abstract
We present detailed theoretical evaluation and thorough experimental investigation of quantitative phase imaging using our previously demonstrated dual-plane in-line digital holographic microscopy technique [Opt. Lett. 35, 3426 (2010)]. This evaluation is based on the recording of two interferograms at slightly different planes and numerically reconstructing the object information. The zero-order diffracted wave is eliminated by using the method of subtraction of average intensity of the entire hologram, and the twin-image diffracted wave is removed by Fourier domain processing of the two recorded holograms. Experiments are performed using controlled amplitude and phase objects and human muscle cells to demonstrate the potential of this technique.
© 2012 Optical Society of America
OCIS Codes
(090.2880) Holography : Holographic interferometry
(100.3010) Image processing : Image reconstruction techniques
(110.0180) Imaging systems : Microscopy
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(090.1995) Holography : Digital holography
(120.5475) Instrumentation, measurement, and metrology : Pressure measurement
ToC Category:
Holography
History
Original Manuscript: September 20, 2011
Revised Manuscript: December 9, 2011
Manuscript Accepted: December 10, 2011
Published: March 16, 2012
Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Bhargab Das, Chandra S. Yelleswarapu, and D. V. G. L. N. Rao, "Quantitative phase microscopy using dual-plane in-line digital holography," Appl. Opt. 51, 1387-1395 (2012)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-51-9-1387
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