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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 52, Iss. 1 — Jan. 1, 2013
  • pp: A216–A222

Three-dimensional subpixel estimation in holographic position measurement of an optically trapped nanoparticle

Akira Sato, Quang Duc Pham, Satoshi Hasegawa, and Yoshio Hayasaki  »View Author Affiliations

Applied Optics, Vol. 52, Issue 1, pp. A216-A222 (2013)

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We propose three-dimensional (3D) subpixel estimation in the position measurement of a nanoparticle held in optical tweezers in water by using an in-line, low-coherence digital holographic microscope. The 3D subpixel estimation was performed with the addition of axial subpixel estimation to the lateral subpixel estimation introduced in our previous work [Appl. Opt. 50, H183 (2011)]. The axial subpixel estimation allowed the step length in the diffraction calculation of a hologram to be increased to 20nm while keeping the axial resolution of 3nm. This drastically decreased the computation time of the diffraction calculation to less than 10% of the two-dimensional subpixel estimation.

© 2012 Optical Society of America

OCIS Codes
(020.7010) Atomic and molecular physics : Laser trapping
(050.1970) Diffraction and gratings : Diffractive optics
(090.1995) Holography : Digital holography
(350.4855) Other areas of optics : Optical tweezers or optical manipulation
(100.4999) Image processing : Pattern recognition, target tracking

Original Manuscript: August 7, 2012
Revised Manuscript: October 7, 2012
Manuscript Accepted: October 7, 2012
Published: November 27, 2012

Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics

Akira Sato, Quang Duc Pham, Satoshi Hasegawa, and Yoshio Hayasaki, "Three-dimensional subpixel estimation in holographic position measurement of an optically trapped nanoparticle," Appl. Opt. 52, A216-A222 (2013)

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