We propose a localized surface plasmon microscope that provides simultaneous imaging of refractive index and fluorescent intensity distributions. We show experimental images of fluorescent and transparent particles under circular pupil illumination to confirm simultaneous high-resolution imaging. Furthermore, we investigate applicability of annular pupil illumination employing two axicons to improve energy efficiency in the fluorescent imaging and find that a brighter image is obtainable by maintaining high spatial resolution for both imaging modes.
© 2013 Optical Society of America
Original Manuscript: January 17, 2013
Revised Manuscript: March 5, 2013
Manuscript Accepted: March 12, 2013
Published: May 7, 2013
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics
Goro Terakado, Jun Ning, Koyo Watanabe, and Hiroshi Kano, "High-resolution simultaneous microscopy of refractive index and fluorescent intensity distributions by using localized surface plasmons," Appl. Opt. 52, 3324-3328 (2013)