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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 52, Iss. 15 — May. 20, 2013
  • pp: 3484–3489

Parallel on-axis phase-shifting holographic phase microscopy based on reflective point-diffraction interferometer with long-term stability

Rongli Guo, Baoli Yao, Peng Gao, Junwei Min, Jun Han, Xun Yu, Ming Lei, Shaohui Yan, Yanlong Yang, Dan Dan, and Tong Ye  »View Author Affiliations

Applied Optics, Vol. 52, Issue 15, pp. 3484-3489 (2013)

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Parallel on-axis two-step phase-shifting reflective point-diffraction interferometry for holographic phase microscopy based on Michelson architecture is proposed. A cube beamsplitter splits the object wave into two copies within the two arms. The reference wave is rebuilt by low-pass filtering with a pinhole-masked mirror. Both object and reference waves are split into two beams by a grating in a 4f imaging system; thus, two interferograms with quadrature phase-shift can be acquired simultaneously with the aid of polarization elements. The approach has the merit of nanometers-scale phase stability over hours due to its quasi-common-path geometry. It can make full use of camera spatial bandwidth while its temporal resolution is as fast as the camera frame rate. Phase imaging on microscale specimen is implemented, and the experimental results demonstrate that the proposed approach is suitable for investigating dynamic processes.

© 2013 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: January 30, 2013
Revised Manuscript: April 12, 2013
Manuscript Accepted: April 17, 2013
Published: May 14, 2013

Virtual Issues
Vol. 8, Iss. 6 Virtual Journal for Biomedical Optics

Rongli Guo, Baoli Yao, Peng Gao, Junwei Min, Jun Han, Xun Yu, Ming Lei, Shaohui Yan, Yanlong Yang, Dan Dan, and Tong Ye, "Parallel on-axis phase-shifting holographic phase microscopy based on reflective point-diffraction interferometer with long-term stability," Appl. Opt. 52, 3484-3489 (2013)

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Fig. 1. Fig. 2. Fig. 3.
Fig. 4.

Supplementary Material

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