Digital in-line holographic microscopy (DIHM) allows access to both intensity and phase information with conventional microscopic lateral resolutions. Such imaging techniques can, however, be used to increase the depth of focus compared to conventional compound microscopes. We present a simple DIHM capable of imaging weakly scattering 10 μm diameter microspheres as well as Hs578T cells over a depth of 1 mm; i.e., we demonstrate an increase by a factor of 100 over the depth of focus of a conventional microscope.
© 2013 Optical Society of America
Original Manuscript: October 26, 2012
Revised Manuscript: January 24, 2013
Manuscript Accepted: January 25, 2013
Published: February 25, 2013
James P. Ryle, Susan McDonnell, Brian Glennon, and John T. Sheridan, "Calibration of a digital in-line holographic microscopy system: depth of focus and bioprocess analysis," Appl. Opt. 52, C78-C87 (2013)