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Applied Optics

Applied Optics


  • Editor: Joseph N. Mait
  • Vol. 53, Iss. 10 — Apr. 1, 2014
  • pp: B181–B191

Laser polarization autofluorescence of endogenous porphyrins of optically anisotropic biological tissues and fluids in diagnostics of necrotic and pathological changes of human organs

Yu. A. Ushenko, A. D. Arkhelyuk, M. I. Sidor, V. T. Bachynskyi, and O. Yu. Wanchuliak  »View Author Affiliations

Applied Optics, Vol. 53, Issue 10, pp. B181-B191 (2014)

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This research presents the results of investigation of laser polarization fluorescence of biological layers (histological sections, cytological smears). The polarization structural properties of autofluorescent images of human biological tissues layers and fluids were found and investigated. A model describing the formation of polarizationally heterogeneous images of optically anisotropic biological layers is suggested. On this basis, the practical method of polarization-variable autofluorescence is analytically substantiated and experimentally tested. The efficiency of applying this method to various tasks of medical diagnostics is analyzed: objectification of histological conclusions, defining and differentiating of various forms of cancer (dysplasia—microinvasive cancer) of the cervix uteri, and forensic medical express-differentiation of cause of death. The objective criteria (statistical moments) of differentiation of autofluorescent images of histological sections of myocardium biopsy and endometrium and cytological smears of its mucous tunic are defined. The operational characteristics (sensitivity, specificity, accuracy) of this method are determined concerning the positions of probative medicine, and the clinical efficiency of the technique is demonstrated.

© 2014 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

Original Manuscript: November 18, 2013
Revised Manuscript: January 23, 2014
Manuscript Accepted: January 23, 2014
Published: March 11, 2014

Virtual Issues
Vol. 9, Iss. 6 Virtual Journal for Biomedical Optics

Yu. A. Ushenko, A. D. Arkhelyuk, M. I. Sidor, V. T. Bachynskyi, and O. Yu. Wanchuliak, "Laser polarization autofluorescence of endogenous porphyrins of optically anisotropic biological tissues and fluids in diagnostics of necrotic and pathological changes of human organs," Appl. Opt. 53, B181-B191 (2014)

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