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Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 53, Iss. 14 — May. 10, 2014
  • pp: 2983–2987

Fabry–Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis

Marco Pisani and Massimo Zucco  »View Author Affiliations


Applied Optics, Vol. 53, Issue 14, pp. 2983-2987 (2014)
http://dx.doi.org/10.1364/AO.53.002983


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Abstract

We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry–Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow–orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0110) Medical optics and biotechnology : Imaging systems
(180.2520) Microscopy : Fluorescence microscopy
(100.3008) Image processing : Image recognition, algorithms and filters
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 3, 2014
Revised Manuscript: March 28, 2014
Manuscript Accepted: March 29, 2014
Published: May 5, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Marco Pisani and Massimo Zucco, "Fabry–Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis," Appl. Opt. 53, 2983-2987 (2014)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-53-14-2983

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