OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Editor: Joseph N. Mait
  • Vol. 53, Iss. 14 — May. 10, 2014
  • pp: 2983–2987

Fabry–Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis

Marco Pisani and Massimo Zucco  »View Author Affiliations


Applied Optics, Vol. 53, Issue 14, pp. 2983-2987 (2014)
http://dx.doi.org/10.1364/AO.53.002983


View Full Text Article

Enhanced HTML    Acrobat PDF (464 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate the ability of our hyperspectral imaging device, based on a scanning Fabry–Perot interferometer, to obtain a single hyper-image of a sample marked with different fluorescent molecules, and to unambiguously discriminate them by observing their spectral fingerprints. An experiment carried out with cyanines, fluorescein, and quantum dots emitting in the yellow–orange region, demonstrates the feasibility of multi-labeled fluorescence microscopy without the use of multiple filter sets or dispersive means.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.0110) Medical optics and biotechnology : Imaging systems
(180.2520) Microscopy : Fluorescence microscopy
(100.3008) Image processing : Image recognition, algorithms and filters
(110.4234) Imaging systems : Multispectral and hyperspectral imaging

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 3, 2014
Revised Manuscript: March 28, 2014
Manuscript Accepted: March 29, 2014
Published: May 5, 2014

Virtual Issues
Vol. 9, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Marco Pisani and Massimo Zucco, "Fabry–Perot-based Fourier-transform hyperspectral imaging allows multi-labeled fluorescence analysis," Appl. Opt. 53, 2983-2987 (2014)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-53-14-2983


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. E. Schröck, S. du Manoir, T. Veldman, B. Schoell, J. Wienberg, M. A. Ferguson-Smith, Y. Ning, D. H. Ledbetter, I. Bar-Am, D. Soenksen, Y. Garini, and T. Ried, “Multicolor spectral karyotyping of human chromosomes,” Science 273, 494–497 (1996). [CrossRef]
  2. M. R. Speicher, S. Gwyn Ballard, and D. C. Ward, “Karyotyping human chromosomes by combinatorial multi-fluor FISH,” Nat. Genet. 12, 368–375 (1996). [CrossRef]
  3. M. Liyanage, A. Coleman, S. du Manoir, T. Veldman, S. McCormack, R. B. Dickson, C. Barlow, A. Wynshaw-Boris, S. Janz, J. Wienberg, M. A. Ferguson-Smith, E. Schröck, and T. Ried, “Multicolour spectral karyotyping of mouse chromosomes,” Nat. Genet. 14, 312–315 (1996). [CrossRef]
  4. T. Ried, M. Liyanage, S. du Manoir, K. Heselmeyer, G. Auer, M. Macville, and E. Schröck, “Tumor cytogenetics revisited: comparative genomic hybridization and spectral karyotyping,” J. Mol. Med. 75, 801–814 (1997).
  5. P. J. Miller and C. C. Hoyt, “Multispectral imaging with a liquid crystal tunable filter,” Proc. SPIE 2345, 354–366 (1995).
  6. E. S. Wachman, W. Niu, and D. L. Farkas, “AOTF microscope for imaging with increased speed and spectral versatility,” Biophys. J. 73, 1215–1222 (1997). [CrossRef]
  7. E. Oh, C. Heo, J. Kim, M. Suh, Y. Lee, and J. Kim, “Hyperspectral fluorescence imaging for cellular iron mapping in the in vitro model of Parkinson’s disease,” J. Biomed. Opt. 19, 051207 (2014). [CrossRef]
  8. D. Cabib, R. A. Buckwald, Y. Garini, N. Katzir, Z. Malik, and D. G. Soeknsen, “Spectral bio-imaging methods for biological research, medical diagnostics and therapy,” U.S. patent5,784,162 (21July1998).
  9. Y. Hiraoka, T. Shimi, and T. Haraguchi, “Multispectral imaging fluorescence microscopy for living cells,” Cell Struct. Funct. 27, 367–374 (2002). [CrossRef]
  10. Y. Garini, I. T. Young, and G. McNamara, “Spectral imaging: principles and applications,” Cytometry A. 69, 735–747 (2006). [CrossRef]
  11. M. Pisani and M. E. Zucco, “Compact imaging spectrometer combining Fourier-transform spectroscopy with a Fabry–Perot interferometer,” Opt. Express 17, 8319–8331 (2009). [CrossRef]
  12. M. Q. Pisani and M. E. Zucco, “Fourier-transform-based hyperspectral imaging,” in Fourier-Transforms—Approach to Scientific Principles, G. Nicolic, ed. (InTech, 2011), pp. 427–446.
  13. M. Zucco and M. Pisani, “Fast processing spectral discrimination for hyperspectral imagers based on interferometry,” Meas. Sci. Technol. 25, 055403 (2014). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited