OSA's Digital Library

Applied Optics

Applied Optics

APPLICATIONS-CENTERED RESEARCH IN OPTICS

  • Vol. 7, Iss. 10 — Oct. 1, 1968
  • pp: 1879–1896

Differential Light Scattering: a Physical Method for Identifying Living Bacterial Cells

Philip J. Wyatt  »View Author Affiliations


Applied Optics, Vol. 7, Issue 10, pp. 1879-1896 (1968)
http://dx.doi.org/10.1364/AO.7.001879


View Full Text Article

Enhanced HTML    Acrobat PDF (2387 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Differential light scattering techniques appear to represent an attractive physical method for the rapid identification of various microorganisms. Certain general results of inverse scattering theory suggest strongly that characteristic of each distinct microorganism that scatters light is an essentially unique scattering pattern, i.e., unique differential scattered intensity and polarization. Although a mathematically rigorous inversion procedure seems impractical at this time, the use of detailed differential scattered intensity data as an identification fingerprint shows considerable. promise. Published measurements on nonbiological scatterers confirm this possibility. A variety of calculations are presented that contrast the expected scattering characteristics of certain microorganisms such as Bacillus subtilis, B. anthracis, Staphylococcus epidermidis, S. aureus, Escherichia coli, and the spores of B. megaterium and B. cereus. Experimental and instrumentation difficulties and possible procedures are discussed. A review and laboration of some applicable features of Rayleigh-Gans scattering are included as an appendix.

© 1968 Optical Society of America

History
Original Manuscript: January 2, 1968
Published: October 1, 1968

Citation
Philip J. Wyatt, "Differential Light Scattering: a Physical Method for Identifying Living Bacterial Cells," Appl. Opt. 7, 1879-1896 (1968)
http://www.opticsinfobase.org/ao/abstract.cfm?URI=ao-7-10-1879

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you wish to use one of your free member downloads to view the figures, click "Enhanced HTML" above and access the figures from the article itself or from the navigation tab.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

If you are accessing the full text through a member bundle, please use the Enhanced HTML link to gain access to the citation lists and other restricted features. Note that accessing both the PDF and HTML versions of an article will count as only one download against your account.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited