Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 40,
  • Issue 5,
  • pp. 696-700
  • (1986)

A Portable Fiberoptic Monitor for Fluorimetric Bioassays

Not Accessible

Your library or personal account may give you access

Abstract

A simple and portable fiberoptic instrument for fluorimetric bioassays is described. The instrument is designed to be readily adaptable to commercially available biotesting wells on microplates. The microwells can also be used as interchangeable sensor heads. The utility of the fiberoptic biosensor is illustrated with a model enzyme-linked immunosorbent assay (ELISA) used to measure varying amounts of rabbit immunoglobulin G from 10 pg to 1 μg. The detection method utilizes an enzyme-amplified immunoassay of 4-methylumbelliferone phosphate as the enzyme substrate. The sensitivity of this assay is three orders of magnitude better than that obtained with a conventional ELISA absorption spectrometric assay using a commercially available reader.

PDF Article
More Like This
Smartphone instrument for portable enzyme- linked immunosorbent assays

Kenneth D. Long, Hojeong Yu, and Brian T. Cunningham
Biomed. Opt. Express 5(11) 3792-3806 (2014)

Plasmonic amplification for bioassays with epi-fluorescence readout

Martin Bauch, Simone Hageneder, and Jakub Dostalek
Opt. Express 22(26) 32026-32038 (2014)

High efficiency ring-lens supercritical angle fluorescence (SAF) detection for optimum bioassay performance

Dirk Kurzbuch, Martin Somers, and Colette McDonagh
Opt. Express 21(19) 22070-22075 (2013)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved