Abstract

The light scattered from translucent samples is used to obtain absorption-type spectra and provide quantitation of an absorber within the scattering bodies. Synthetic vesicles with an encapsulated dye, bromo-thymol blue (BTB), are employed as a novel model system to study the properties of translucent samples. Chlorophyll measurement in intact phytoplankton and algae is demonstrated. A least-squares fitting procedure is used to overcome the effect of chlorophyll fluorescence on the scattering data. A straight line relationship between a defined pseudo-absorbance and concentration is shown for both the BTB and chlorophyll data. A limit of detection of about 0.2 mg/L BTB is obtained for the synthetic vesicles. For chlorophyll the limit of detection is in the range of 1 to 5% of that found in the native plankton and algae samples.

Optical dynamic deformation measurements at translucent materials

Katrin Philipp, Nektarios Koukourakis, Robert Kuschmierz, Christoph Leithold, Andreas Fischer, and Jürgen Czarske
Opt. Lett. 40(4) 514-517 (2015)

Evaluating edge loss in the reflectance measurement of translucent materials

Lou Gevaux, Lionel Simonot, Raphael Clerc, Morgane Gerardin, and Mathieu Hebert
Appl. Opt. 59(28) 8939-8950 (2020)

Statistical approach for the retrieval of phytoplankton community structures from in situ fluorescence measurements

Shengqiang Wang, Cong Xiao, Joji Ishizaka, Zhongfeng Qiu, Deyong Sun, Qian Xu, Yuanli Zhu, Yu Huan, and Yuji Watanabe
Opt. Express 24(21) 23635-23653 (2016)

Frequency Response Method of High Speed Spectrophotometry for Luminescent Materials

K. Doi and A. Toshinai
Appl. Opt. 9(12) 2762-2767 (1970)

Modeling the inherent optical properties of the ocean based on the detailed composition of the planktonic community

Dariusz Stramski, Annick Bricaud, and André Morel
Appl. Opt. 40(18) 2929-2945 (2001)