General advantages and potential limitations of Fourier transform (FT) Raman spectroscopy using Nd:YAG laser excitation at 1064 nm have been considered for both routine analysis and specific biophysical applications. Optical design and operating parameters which affect the quality and reproducibility of the data are discussed. Moderately high resolution spectra (0.25 cm⁻¹) of liquids are obtained with relative ease, and the results are compared with dispersive spectra. Particular emphasis has been placed on applications to biological systems where intrinsic fluorescence has traditionally limited the use of dispersive Raman spectroscopy. As an example of a biophysical study, we demonstrate the utility of FT-Raman spectroscopy in elucidating the interactions of polyene antibiotics with model membrane lipid bilayers as a means of understanding novel drug/membrane interactions at the molecular level.

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription