Abstract

A computerized method is described for correcting systematic distortion of images from slab gel electrophoresis. Such distortions can lead to misinterpretation of the information contained in the gel. The method is useful for data analysis in one-dimension slab gel electrophoresis where the information is manifested in rectangular shaped bands, such as conventional restriction digest or sequencing gels, and the distortions can be adequately described by continuous low-order polynomial functions. The purpose is to eliminate human skill and judgement from the process and to minimize human interaction, which would be useful in any future attempts to automate the analysis of electrophoretic gels.

Quantitative estimation of crazing in sol-gel layers by automated optical microscopy analysis.

J. Avice, C. Boscher, P. Voarino, G. Brotons, and H. Piombini
Opt. Express 25(23) 28851-28869 (2017)

Automated bead-trapping apparatus and control system for single-molecule DNA sequencing

Greg Bashford, Don Lamb, Dan Grone, Bob Eckles, Kevin Kornelsen, Lyle Middendorf, and John Williams
Opt. Express 16(5) 3445-3455 (2008)

Accommodating brightness and exposure levels in densitometry of stained polyacrylamide electrophoresis gels

Han Yen Tan, Tuck Wah Ng, and Oi Wah Liew
Appl. Opt. 49(9) 1623-1629 (2010)

Automated image analysis in the diagnosis of bladder cancer

Karl K. Melder and Leopold G. Koss
Appl. Opt. 26(16) 3367-3372 (1987)

Optical flow computation in combustion image sequences

Robin N. Strickland and Donald W. Sweeney
Appl. Opt. 27(24) 5213-5220 (1988)