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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 63, Iss. 5 — May. 1, 2009
  • pp: 528–537

Probing Water and Biomolecules at the Air–Water Interface with a Broad Bandwidth Vibrational Sum Frequency Generation Spectrometer from 3800 to 900 cm−1

Gang Ma, Jian Liu, Li Fu, and Elsa C. Y. Yan

Applied Spectroscopy, Vol. 63, Issue 5, pp. 528-537 (2009)


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Abstract

We have built a broad bandwidth vibrational sum frequency generation (VSFG) spectrometer that can provide high-quality spectra over the range of 3800 to 900 cm<sup>−1</sup>. The spectrometer contains a commercial Ti:sapphire based 6 W regenerative amplifier as the master light source, a home-built pulse shaper to produce a narrow bandwidth 800 nm beam, a commercial optical parametric amplifier to generate a broad bandwidth femtosecond infrared (IR) pulse, and a detection system with a monochromator and a charge-coupled device (CCD). We applied this spectrometer to obtain VSFG spectra of a lipid monolayer at the air–water interface in the O–H stretching region (3800–3000 cm<sup>−1</sup>), the C–H stretching region (3100–2700 cm<sup>−1</sup>), the C–D stretching region (2300–2000 cm<sup>−1</sup>), the C=O stretching region (1800–1700 cm<sup>−1</sup>), and the PO<sub>2</sub><sup>−</sup> symmetric stretching region (1200–1000 cm<sup>−1</sup>). We also obtained the VSFG spectrum of neat water in the O–H stretching region (3800–3000 cm<sup>−1</sup>) and the VSFG spectrum of a protein, α-synuclein, in the amide I region (1700–1600 cm<sup>−1</sup>) at the air–water interface. The spectrometer can provide a VSFG spectrum in the O–H stretching region (3800–3000 cm<sup>−1</sup>) without scanning the IR frequency. This feature will be useful in probing water dynamics at interfaces because the free OH and H-bonded OH can be investigated simultaneously. We have also provided instrumental details and discussed further improvements that should be beneficial to other researchers interested in setting up VSFG instrumentation.

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Citation
Gang Ma, Jian Liu, Li Fu, and Elsa C. Y. Yan, "Probing Water and Biomolecules at the Air–Water Interface with a Broad Bandwidth Vibrational Sum Frequency Generation Spectrometer from 3800 to 900 cm−1," Appl. Spectrosc. 63, 528-537 (2009)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-63-5-528


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