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Applied Spectroscopy

Applied Spectroscopy

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  • Vol. 65, Iss. 4 — Apr. 1, 2011
  • pp: 115A–124A

Fluorescence Correlation Spectroscopy: A Review of Biochemical and Microfluidic Applications

Yu Tian, Michelle M. Martinez, and Dimitri Pappas

Applied Spectroscopy, Vol. 65, Issue 4, pp. 115A-124A (2011)


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Abstract

Over the years fluorescence correlation spectroscopy (FCS) has proven to be a useful technique that has been utilized in several fields of study. Although FCS initially suffered from poor signal-to-noise ratios, the incorporation of confocal microscopy has overcome this drawback and transformed FCS into a sensitive technique with high figures of merit. In addition, tandem methods have evolved to include dual-color cross-correlation, total internal reflection fluorescence correlation, and fluorescence lifetime correlation spectroscopy combined with time-correlated single-photon counting. In this review, we discuss several applications of FSC for biochemical, microfluidic, and cellular investigations.

Virtual Issues
(2011) Advances in Optics and Photonics
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Yu Tian, Michelle M. Martinez, and Dimitri Pappas, "Fluorescence Correlation Spectroscopy: A Review of Biochemical and Microfluidic Applications," Appl. Spectrosc. 65, 115A-124A (2011)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-65-4-115A


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