OSA's Digital Library

Applied Spectroscopy

Applied Spectroscopy

| PUBLISHED BY SAS — AVAILABLE FROM SAS AND OSA

  • Vol. 67, Iss. 2 — Feb. 1, 2013
  • pp: 196–203

Multiwavelength Transmission Spectroscopy Revisited for the Characterization of the Protein and Polystyrene Nanoparticle Mixtures

Yulia M. Serebrennikova, Alison Roth, Debra E. Huffman, Jennifer M. Smith, Jack N. Lindon, and Luis H. García-Rubio

Applied Spectroscopy, Vol. 67, Issue 2, pp. 196-203 (2013)


View Full Text Article

Acrobat PDF (1707 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Multiwavelength Transmission (MWT) UV-Vis-NIR spectroscopy, an effective technique often underutilized for the characterization of processes involving particulates, such as protein aggregation, is systematically explored using bovine serum albumin and a set of NIST-traceable particle size (PS) standards having certified particle diameters over the nominal size range of 30 to 100 nm. The PS standards are used as surrogates for protein aggregates and other contaminants such as oils and microbubbles. Therefore, the standards can be used to quantitatively modify the optical properties of protein solutions and thus observe the effect of the presence of aggregates and other particulates on their wavelength-dependent transmission spectra. The experimental results demonstrate that the changes induced in the optical density spectra of proteins due to the presence of PS particles are detectable and consistent with the expectations set by light scattering theory. It is demonstrated that the size and relative concentrations of the particle populations present in the protein samples can be quantified. Because of the considerable dynamic range of MWT UV-Vis-NIR spectroscopy for particle analysis and its real-time measurement capabilities, this type of spectroscopy can be effectively used for the characterization of protein aggregates and for the continuous real-time monitoring of aggregation processes and for the identification and quantification of contaminants in protein-based products.

Virtual Issues
Vol. 8, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Yulia M. Serebrennikova, Alison Roth, Debra E. Huffman, Jennifer M. Smith, Jack N. Lindon, and Luis H. García-Rubio, "Multiwavelength Transmission Spectroscopy Revisited for the Characterization of the Protein and Polystyrene Nanoparticle Mixtures," Appl. Spectrosc. 67, 196-203 (2013)
http://www.opticsinfobase.org/as/abstract.cfm?URI=as-67-2-196


Sort:  Journal  |  Reset

References

References are not available for this paper.

Cited By

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited